Recombinant Anti-FAM111A antibody [EPR14407] (ab184572)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14407] to FAM111A
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FAM111A antibody [EPR14407]
See all FAM111A primary antibodies -
Description
Rabbit monoclonal [EPR14407] to FAM111A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- SW480, BxPC-3 or T47-D cell lysates; T47-D cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14407 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
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ICC/IF |
1/100.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
ICC/IF
1/100. |
Target
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Relevance
This protein of unknown function belongs to the FAM111 family. -
Database links
- Entrez Gene: 63901 Human
- Omim: 615292 Human
- SwissProt: Q96PZ2 Human
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Alternative names
- Family with sequence similarity 111 member A antibody
- GCLEB antibody
- Hypothetical protein LOC63901 antibody
see all
Images
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All lanes : Anti-FAM111A antibody [EPR14407] (ab184572) at 1/1000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : BxPC-3 cell lysate
Lane 3 : T47-D cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa -
Immunofluorescent analysis of 4% paraformaldehyde-fixed T47-D cells labeling FAM111A with ab184572 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/1200 dilution (red). Counter stained with Dapi (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab184572 has been referenced in 5 publications.
- Nie M et al. FAM111A induces nuclear dysfunction in disease and viral restriction. EMBO Rep 22:e50803 (2021). PubMed: 33369867
- Kojima Y et al. FAM111A protects replication forks from protein obstacles via its trypsin-like domain. Nat Commun 11:1318 (2020). PubMed: 32165630
- Hoffmann S et al. FAM111 protease activity undermines cellular fitness and is amplified by gain-of-function mutations in human disease. EMBO Rep 21:e50662 (2020). PubMed: 32776417
- Tarnita RM et al. Contribution of DNA Replication to the FAM111A-Mediated Simian Virus 40 Host Range Phenotype. J Virol 93:N/A (2019). PubMed: 30333173
- Panda D et al. Triad of human cellular proteins, IRF2, FAM111A, and RFC3, restrict replication of orthopoxvirus SPI-1 host-range mutants. Proc Natl Acad Sci U S A 114:3720-3725 (2017). PubMed: 28320935