Overview

  • Product name

  • Description

    Rabbit polyclonal to FBXL3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 14 and 279 of Human FBXL3 (NP_036290).

  • Positive control

    • 293T, H1299, HeLa, HepG2 and Raji cell lines. MOLT4 whole cell lysate and A431 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab96645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Substrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex involved in circadian clock function. The SCF(FBXL3) complex acts by mediating ubiquitination and subsequent degradation of CRY1 and CRY2. Recruiter of target protein that may recognize and bind to some phosphorylated proteins and promotes their ubiquitination and degradation.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Contains 1 F-box domain.
    Contains 7 LRR (leucine-rich) repeats.
  • Cellular localization

    Nucleus. Cytoplasm. Predominantly nuclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • F box and leucine rich repeat protein 3 antibody
    • F box and leucine rich repeat protein 3A antibody
    • F box protein Fbl3a antibody
    • F-box and leucine-rich repeat protein 3A antibody
    • F-box/LRR-repeat protein 3 antibody
    • F-box/LRR-repeat protein 3A antibody
    • FBL3 antibody
    • FBL3A antibody
    • Fbxl3 antibody
    • FBXL3_HUMAN antibody
    • FBXL3A antibody
    see all

Images

  • Anti-FBXL3 antibody (ab96645) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 49 kDa



    10% SDS PAGE
  • ab96645, at a 1/200 dilution, staining FBXL3 in paraformaldehyde fixed A431 by Immunofluorescence analysis. Lower image merged with DNA probe.

References

This product has been referenced in:

  • Toledo M  et al. Autophagy Regulates the Liver Clock and Glucose Metabolism by Degrading CRY1. Cell Metab 28:268-281.e4 (2018). Read more (PubMed: 29937374) »
  • Wang D  et al. FBXL3 is regulated by miRNA-4735-3p and suppresses cell proliferation and migration in non-small cell lung cancer. Pathol Res Pract N/A:N/A (2018). Read more (PubMed: 30594330) »
See all 2 Publications for this product

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