Anti-FGF 23 antibody (ab56326)
Key features and details
- Goat polyclonal to FGF 23
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FGF 23 antibody
See all FGF 23 primary antibodies -
Description
Goat polyclonal to FGF 23 -
Host species
Goat -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
C-RHTRSAEDDSERD
, corresponding to internal sequence amino acids 176-188 of Human FGF 23 -
Positive control
- Human Brain (Hippocampus) lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab56326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 28 kDa).
A 1 hour primary incubation is recommended for this product. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide. |
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ICC/IF |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 28 kDa). A 1 hour primary incubation is recommended for this product. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide. |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. -
Tissue specificity
Expressed in osteogenic cells particularly during phases of active bone remodeling. In adult trabecular bone, expressed in osteocytes and flattened bone-lining cells (inactive osteoblasts). -
Involvement in disease
Defects in FGF23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) [MIM:193100]. ADHR is characterized by low serum phosphorus concentrations, rickets, osteomalacia, leg deformities, short stature, bone pain and dental abscesses.
Defects in FGF23 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Post-translational
modificationsFollowing secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases.
O-glycosylated by GALT3. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23. -
Cellular localization
Secreted. Secretion is dependent on O-glycosylation. - Information by UniProt
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Database links
- Entrez Gene: 8074 Human
- Omim: 605380 Human
- SwissProt: Q9GZV9 Human
- Unigene: 287370 Human
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Alternative names
- ADHR antibody
- FGF-23 antibody
- Fgf23 antibody
see all
Images
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Anti-FGF 23 antibody (ab56326) at 0.5 µg/ml + Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer)
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence. -
ICC/IF image of ab56326 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56326, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (10)
ab56326 has been referenced in 10 publications.
- Fang L et al. FGF23 promotes proliferation, migration and invasion by regulating miR-340-5p in osteosarcoma. J Orthop Surg Res 18:12 (2023). PubMed: 36604721
- Leow MKS et al. Paraneoplastic Secretion of Multiple Phosphatonins From a Deep Fibrous Histiocytoma Causing Oncogenic Osteomalacia. J Clin Endocrinol Metab 106:e2299-e2308 (2021). PubMed: 33462615
- Ursem SR et al. Localization of fibroblast growth factor 23 protein in the rat hypothalamus. Eur J Neurosci 54:5261-5271 (2021). PubMed: 34184338
- Li D et al. Mg-Zn-Mn alloy extract induces the angiogenesis of human umbilical vein endothelial cells via FGF/FGFR signaling pathway. Biochem Biophys Res Commun 514:618-624 (2019). PubMed: 31076107
- Xu X et al. Dysregulated systemic lymphocytes affect the balance of osteogenic/adipogenic differentiation of bone mesenchymal stem cells after local irradiation. Stem Cell Res Ther 8:71 (2017). PubMed: 28320453
- Mace ML et al. Kidney fibroblast growth factor 23 does not contribute to elevation of its circulating levels in uremia. Kidney Int 92:165-178 (2017). PubMed: 28341272
- Zou Q et al. Bone marrow stem cell dysfunction in radiation-induced abscopal bone loss. J Orthop Surg Res 11:3 (2016). WB . PubMed: 26739584
- Guo C et al. Increased EZH2 and decreased osteoblastogenesis during local irradiation-induced bone loss in rats. Sci Rep 6:31318 (2016). WB . PubMed: 27499068
- Uchihashi K et al. Phosphaturic mesenchymal tumor, nonphosphaturic variant, causing fatal pulmonary metastasis. Hum Pathol 44:2614-8 (2013). PubMed: 23954138
- Tang WJ et al. Autocrine/paracrine action of vitamin D on FGF23 expression in cultured rat osteoblasts. Calcif Tissue Int 86:404-10 (2010). PubMed: 20354682