Anti-FGF17 antibody (ab9990)

Submit a review Q&A (3)

Overview

  • Product name
    Anti-FGF17 antibody
    See all FGF17 primary antibodies
  • Description
    Rabbit polyclonal to FGF17
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, Neutralising, WB, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hFGF-17 (human Fibroblast Growth Factor-17)

  • Positive control
    • Recombinant human FGF17 protein (ab9991) can be used as a positive control in WB.

Properties

  • Form
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml. (using 100µl/well antibody solution). Allows the detection of 0.2 - 0.4 ng/well of recombinant hFGF-17.
Neutralising Use a concentration of 0.02 - 0.035 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of hFGF-17 (1.5 ng/ml).
WB Use a concentration of 0.1 - 0.2 µg/ml.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [MM0284-5H27] to FGF17 (ab89270).

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab89270 as Capture.

Target

  • Function
    Plays an important role in the regulation of embryonic development and as signaling molecule in the induction and patterning of the embryonic brain. Required for normal brain development.
  • Tissue specificity
    Preferentially expressed in the embryonic brain.
  • Sequence similarities
    Belongs to the heparin-binding growth factors family.
  • Developmental stage
    Detected in embryos at E14.5, but not at E10.5 and E19.5. Preferentially expressed in the neuroepithelia of the isthmus and septum of the embryonic brain at E14.5.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • FGF 13 antibody
      • FGF 17 antibody
      • FGF-17 antibody
      • FGF13 antibody
      • Fgf17 antibody
      • FGF17_HUMAN antibody
      • Fibroblast growth factor 17 antibody
      • HH20 antibody
      see all

    Images

    References

    ab9990 has not yet been referenced specifically in any publications.

    Publishing research using ab9990? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    Hello, I was wondering if you could tell me smallest amount of FGF-17 detectable in a western blot using Ab9990 for detection? Thank You!

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    Answer

    Thank yoiu for your enquiry. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-17 using ab9990 FGF17 antibody is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Some lots may be more sensitive than this. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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    Question

    Thank you for your quick reply! Since I am planning on trying the antibody on chicken or mouse proteins, and we do not know whether it would recognize them, I wonder whether I could ask for a sample size to try or to purchase. Thanks, and have a nice day!

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    Answer

    Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abpoints which can be redeemed on a number of rewards (a further 100 Abpoints will be offered for an image). Please contact us again if you have any additional questions, and happy holidays.

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    Question

    I wonder whether this anti-Fgf17 antibody may cross react with Fgf17 in chicken and mice. In addition, could you provide any references when people tried to use it for Western blot?

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    Answer

    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with chicken and mouse have not yet been tested for use with ab9990. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 points will be offered for an image). Presently, we're not aware of any publications which feature the use of this antibody. Please rest assured that our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Please contact us again if you have any additional questions.

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