Anti-FGF17 antibody (ab9990)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-FGF17 antibody
See all FGF17 primary antibodies -
DescriptionRabbit polyclonal to FGF17
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Host speciesRabbit
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Tested applicationsSuitable for: ELISA, Neutralising, WB, Sandwich ELISAmore details
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Species reactivityReacts with: Human
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Immunogen
Highly pure (>98%) recombinant hFGF-17 (human Fibroblast Growth Factor-17)
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Positive control
- Recombinant human FGF17 protein (ab9991) can be used as a positive control in WB.
Properties
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FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading...
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PurityImmunogen affinity purified
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ClonalityPolyclonal
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Isotypeunknown
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab9990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA | Use a concentration of 0.5 µg/ml. (using 100µl/well antibody solution). Allows the detection of 0.2 - 0.4 ng/well of recombinant hFGF-17. | |
Neutralising | Use a concentration of 0.02 - 0.035 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of hFGF-17 (1.5 ng/ml). | |
WB | Use a concentration of 0.1 - 0.2 µg/ml. | |
Sandwich ELISA | Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [MM0284-5H27] to FGF17 (ab89270). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab89270 as Capture. |
Target
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FunctionPlays an important role in the regulation of embryonic development and as signaling molecule in the induction and patterning of the embryonic brain. Required for normal brain development.
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Tissue specificityPreferentially expressed in the embryonic brain.
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Sequence similaritiesBelongs to the heparin-binding growth factors family.
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Developmental stageDetected in embryos at E14.5, but not at E10.5 and E19.5. Preferentially expressed in the neuroepithelia of the isthmus and septum of the embryonic brain at E14.5.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 8822 Human
- Omim: 603725 Human
- SwissProt: O60258 Human
- Unigene: 248192 Human
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Alternative names
- FGF 13 antibody
- FGF 17 antibody
- FGF-17 antibody
see all
Images
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Standard Curve for FGF17 (Analyte: ab9991) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MM0284-5H27] to FGF17 (ab89270) at 1ug/ml and Detector Antibody Rabbit polyclonal to FGF17 (ab9990) at 0.5ug/ml
Protocols
Datasheets and documents
References
ab9990 has not yet been referenced specifically in any publications.