Overview

  • Product name
  • Description
    Rabbit polyclonal to FOLR2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FOLR2.

    Read Abcam's proprietary immunogen policy (Peptide available as ab104497.)

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Spleen; Bone marrow; Thymus

Properties

Applications

Our Abpromise guarantee covers the use of ab103998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).

Target

  • Function
    Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells.
  • Tissue specificity
    Expressed in placenta and hematopoietic cells. Expression is increased in malignant tissues.
  • Sequence similarities
    Belongs to the folate receptor family.
  • Post-translational
    modifications
    Eight disulfide bonds are present.
    N-glycosylated.
  • Cellular localization
    Cell membrane. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • beta hFR antibody
    • FBP antibody
    • FBP/PL 1 antibody
    • FBP2 antibody
    • fetal/placental antibody
    • folate receptor 2 (fetal) antibody
    • Folate receptor 2 antibody
    • Folate receptor antibody
    • Folate receptor beta antibody
    • Folate receptor, fetal/placental antibody
    • Folbp 2 antibody
    • Folbp2 antibody
    • Folr2 antibody
    • FOLR2_HUMAN antibody
    • FR beta antibody
    • FR P3 antibody
    • FR-beta antibody
    • Placental folate binding protein antibody
    • Placental folate-binding protein antibody
    see all

Images

  • All lanes : Anti-FOLR2 antibody (ab103998) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate - total protein (ab29699)
    Lane 2 : Bone marrow (Human) Tissue Lysate
    Lane 3 : Human thymus tissue lysate - total protein (ab30146)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 32 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    FOLR2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ICC/IF image of ab103998 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103998 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Hek293, HepG2, and MCF-7 formaldehyde fixed cells at 5ug/ml and also in HeLa, Hek293, HepG2, and MCF-7 Methanol fixed cells at 5ug/ml.

References

ab103998 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

DISCOUNT CODE: ***
Expiration date: February 1, 2013

I am very pleased to hear you would like to accept our offer and testab103998 in rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forrat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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