Anti-FOXA2 antibody [EPR4466] (ab108422)
- Datasheet
- References (15)
- Protocols
Overview
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Product nameAnti-FOXA2 antibody [EPR4466]
See all FOXA2 primary antibodies -
DescriptionRabbit monoclonal [EPR4466] to FOXA2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human FOXA2 aa 350-450. The exact sequence is proprietary.
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Positive control
- WB: Human colon cancer, fetal colon and mouse lung tissue lysates and HepG2 whole cell lysate (ab7900). IHC-P: Human hepatocellular carcinoma and mouse liver tissue; Human colon tissue; Mouse and rat brain (substantia nigra) tissue. ICC/IF: HT-29 cells.
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General notes
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR4466
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IsotypeIgG
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Research areas
Associated products
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Alternative Products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
- HepG2 cytoplasmic extract lysate (ab14659)
- HepG2 nuclear extract lysate (ab14660)
- Mouse lung normal tissue lysate - total protein (ab29297)
- Mouse lung tissue lysate - total protein (0 days) (ab7267)
- Mouse lung tissue lysate - total protein (14 days) (ab7268)
- Mouse lung tissue lysate - total protein (7 days) (ab7270)
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Related Products
Applications
Our Abpromise guarantee covers the use of ab108422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| WB | 1/1000. Predicted molecular weight: 52 kDa. For unpurified use at 1/1000 - 1/10000. |
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| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
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| ICC/IF | 1/300. For unpurified use at 1/250 - 1/500. |
Target
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FunctionTranscription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
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Sequence similaritiesContains 1 fork-head DNA-binding domain.
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Post-translational
modificationsPhosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation. -
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 3170 Human
- Entrez Gene: 15376 Mouse
- Entrez Gene: 25099 Rat
- Omim: 600288 Human
- SwissProt: Q9Y261 Human
- SwissProt: P35583 Mouse
- SwissProt: P32182 Rat
- Unigene: 155651 Human
see all -
Alternative names
- Forkhead box A2 antibody
- Forkhead box protein A2 antibody
- FOX A2 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.com/ps/products/108/ab108422/Images/ab108422-242449-ab108422iccif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4466] (ab108422)Immunocytochemistry/Immunofluorescence analysis of HT-29 cells labelling FOXA2 with purified ab108422 at 1/300. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.
-ve control 1: primary antibody (1/300) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
-ve control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.com/ps/products/108/ab108422/Images/ab108422-242447-ab108422ihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling FOXA2 with purified ab108422 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.com/ps/products/108/ab108422/Images/FOXA2-Primary-antibodies-ab108422-1.jpg)
Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)All lanes : Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal colon tissue lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 52 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.com/ps/products/108/ab108422/Images/ab108422-242448-ab108422ihcp2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling FOXA2 with purified ab108422 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.com/ps/products/108/ab108422/Images/ab108422-242445-ab108422wb.jpg)
Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/10000 dilution (purified) + Human colon cancer tissue lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.com/ps/products/108/ab108422/Images/ab108422-267986-ab108422IHCP.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)This image is courtesy of Carl Hobbs, King's College London, United KingdomImmunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human colon sections labelling FOXA2 with ab108422 at dilution of 1/500. The secondary antibody used was a polyclonal goat anti-rabbit biotin conjugated antibody at a dilution of 1/300. The sample was counterstained with hematoxylin. Antigen retrieval was heat mediated using citric acid.
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![Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.com/ps/products/108/ab108422/Images/ab108422-242446-ab108422wb2.jpg)
Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/2000 dilution (purified) + Mouse lung tissue lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.com/ps/products/108/ab108422/Images/FOXA2-Primary-antibodies-ab108422-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling FOXA2 with unpurified ab108422 at a 1/250 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.com/ps/products/108/ab108422/Images/ab108422-264049-FOXA2-ab108422-MS-substantia-nigra-1in-500.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)This image is courtesy of Carl Hobbs, King's College London, United Kingdomab108422 staining of FOXA2 in mouse brain (substantia nigra) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/500) for two hours at room temperature. A Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.com/ps/products/108/ab108422/Images/ab108422-264050-FOXA2-ab108422-RAT-substantia-nigra-1in-500.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)This image is courtesy of Carl Hobbs, King's College London, United Kingdomab108422 staining of FOXA2 in rat brain (substantia nigra) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/500) for two hours at room temperature. A Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Morgani SM et al. Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning. Elife 7:N/A (2018). Read more (PubMed: 29412136) »
- Shafa M et al. Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers. Front Med (Lausanne) 5:69 (2018). Read more (PubMed: 29600249) »
