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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box FOXA
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RecombinantRabMAb

Recombinant Anti-FOXA2 antibody [EPR4466] (ab108422)

  • Datasheet
  • SDS
Reviews (7) Submit a question References (42)

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Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4466] (ab108422)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)
  • Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)
  • Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)
  • Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)
  • Anti-FOXA2 antibody [EPR4466] (ab108422)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4466] to FOXA2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-FOXA2 antibody [EPR4466]
    See all FOXA2 primary antibodies
  • Description

    Rabbit monoclonal [EPR4466] to FOXA2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human colon cancer, fetal colon and mouse lung tissue lysates and HepG2 whole cell lysate (ab7900). IHC-P: Human hepatocellular carcinoma and mouse liver tissue; Human colon tissue; Mouse brain (substantia nigra) tissue. ICC/IF: HT-29 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4466
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • FOXA
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-FOXA2 antibody [EPR4466] (ab193864)
    • Alexa Fluor® 647 Anti-FOXA2 antibody [EPR4466] (ab193879)
    • HRP Anti-FOXA2 antibody [EPR4466] (ab193880)
    • Anti-FOXA2 antibody [EPR4466] - BSA and Azide free (ab220810)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 cytoplasmic extract lysate (ab14659)
    • Hep G2 nuclear extract lysate (ab14660)
    • Mouse lung normal tissue lysate - total protein (ab29297)
    • Mouse lung tissue lysate - total protein (0 days) (ab7267)
    • Mouse lung tissue lysate - total protein (14 days) (ab7268)
    • Mouse lung tissue lysate - total protein (7 days) (ab7270)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Mouse
Human
WB
Mouse
Human
All applications
Rat
Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 52 kDa.

For unpurified use at 1/1000 - 1/10000.

IHC-P (4)
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/250 - 1/500.

ICC/IF
1/300.

For unpurified use at 1/250 - 1/500.

Notes
WB
1/1000. Predicted molecular weight: 52 kDa.

For unpurified use at 1/1000 - 1/10000.

IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/250 - 1/500.

ICC/IF
1/300.

For unpurified use at 1/250 - 1/500.

Target

  • Function

    Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications

    Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
  • Cellular localization

    Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.
  • Target information above from: UniProt accession Q9Y261 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3170 Human
    • Entrez Gene: 15376 Mouse
    • Entrez Gene: 25099 Rat
    • Omim: 600288 Human
    • SwissProt: Q9Y261 Human
    • SwissProt: P35583 Mouse
    • SwissProt: P32182 Rat
    • Unigene: 155651 Human
    • Unigene: 938 Mouse
    • Unigene: 10948 Rat
    see all
  • Alternative names

    • Forkhead box A2 antibody
    • Forkhead box protein A2 antibody
    • FOX A2 antibody
    • foxa2 antibody
    • FOXA2_HUMAN antibody
    • Hepatic nuclear factor 3 beta antibody
    • Hepatocyte nuclear factor 3 antibody
    • Hepatocyte nuclear factor 3 beta antibody
    • Hepatocyte nuclear factor 3-beta antibody
    • HNF 3B antibody
    • HNF-3-beta antibody
    • HNF-3B antibody
    • HNF3B antibody
    • MGC19807 antibody
    • TCF 3B antibody
    • TCF-3B antibody
    • TCF3B antibody
    • Transcription factor 3B antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4466] (ab108422)

    Immunocytochemistry/Immunofluorescence analysis of HT-29 cells labelling FOXA2 with purified ab108422 at 1/300. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    -ve control 1: primary antibody (1/300) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    -ve control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling FOXA2 with purified ab108422 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)
    All lanes : Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal colon tissue lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG

    Predicted band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling FOXA2 with purified ab108422 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/10000 dilution (purified) + Human colon cancer tissue lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)This image is courtesy of Carl Hobbs, King's College London, United Kingdom

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human colon sections labelling FOXA2 with ab108422 at dilution of 1/500. The secondary antibody used was a polyclonal goat anti-rabbit biotin conjugated antibody at a dilution of 1/300. The sample was counterstained with hematoxylin. Antigen retrieval was heat mediated using citric acid.

    See Abreview

  • Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/2000 dilution (purified) + Mouse lung tissue lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling FOXA2 with unpurified ab108422 at a 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)This image is courtesy of Carl Hobbs, King's College London, United Kingdom

    ab108422 staining of FOXA2 in mouse brain (substantia nigra) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/500) for two hours at room temperature. A Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

  • Anti-FOXA2 antibody [EPR4466] (ab108422)
    Anti-FOXA2 antibody [EPR4466] (ab108422)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (42)

Publishing research using ab108422? Please let us know so that we can cite the reference in this datasheet.

ab108422 has been referenced in 42 publications.

  • Poncet N  et al. Wnt regulates amino acid transporter Slc7a5 and so constrains the integrated stress response in mouse embryos. EMBO Rep 21:e48469 (2020). PubMed: 31789450
  • Hong CT  et al. Insulin Resistance Promotes Parkinson's Disease through Aberrant Expression of a-Synuclein, Mitochondrial Dysfunction, and Deregulation of the Polo-Like Kinase 2 Signaling. Cells 9:N/A (2020). PubMed: 32192190
  • Jefferson WN  et al. Uterine Patterning, Endometrial Gland Development, and Implantation Failure in Mice Exposed Neonatally to Genistein. Environ Health Perspect 128:37001 (2020). PubMed: 32186404
  • Kim YY  et al. Effects of Natural Progesterone and Synthetic Progestin on Germ Layer Gene Expression in a Human Embryoid Body Model. Int J Mol Sci 21:N/A (2020). PubMed: 31991577
  • Thanan R  et al. Opposing Roles of FoxA1 and FoxA3 in Intrahepatic Cholangiocarcinoma Progression. Int J Mol Sci 21:N/A (2020). PubMed: 32151057
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-7 of 7 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-FOXA2 antibody [EPR4466]

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E12.5 Mouse Brain section (Brainstem))
Specification
E12.5 Mouse Brain section (Brainstem)
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Dr. Alain Chedotal

Verified customer

Submitted Feb 25 2021

ChIP abreview for Anti-FOXA2 antibody [EPR4466]

Excellent
Abreviews
Abreviews
Application
ChIP
Sample
Human Cell lysate - nuclear (Prostate cancer cells)
Negative control
FOXA2 overexpression prostate cancer cells, IgG ChIP. KLK3 enhancer region
Specification
Prostate cancer cells
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control
FOXA2 overexpression prostate cancer cells, FoxA2 ChIP. KLK3 enhancer region
Read More

Abcam user community

Verified customer

Submitted Oct 07 2019

IHC - Wholemount abreview for Anti-FOXA2 antibody [EPR4466]

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Embryo (E8.5 mouse embryo)
Specification
E8.5 mouse embryo
Read More

Ms. Heather Wilson

Verified customer

Submitted Aug 15 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXA2 antibody [EPR4466]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Baboon Tissue sections (Endometriotic Lesion)
Antigen retrieval step
Other
Permeabilization
No
Specification
Endometriotic Lesion
Blocking step
Normal Horse Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
10% Buffered Formalin
Read More

Abcam user community

Verified customer

Submitted Sep 09 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXA2 antibody [EPR4466]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Lung
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Mr. Carl Hobbs

Verified customer

Submitted Sep 06 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXA2 antibody [EPR4466]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Mr. Carl Hobbs

Verified customer

Submitted Sep 06 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXA2 antibody [EPR4466]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Colon
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Mr. Carl Hobbs

Verified customer

Submitted Sep 06 2016

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