Overview

  • Product name
  • Description
    Rabbit polyclonal to FOXP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
    Predicted to work with: Dog, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human FOXP1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab20276.)

  • Positive control
    • This antibody gave a positive signal in Jurkat nuclear lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab16645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/200 - 1/500. PubMed: 20364144
IP Use at an assay dependent concentration.
WB 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).

ab16645 recognises the different isoforms of FOXP1 in Jurkat cell lysate (see review for further details).

IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/500.

Target

  • Function
    Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development.
  • Involvement in disease
    Note=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
    Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors.
  • Sequence similarities
    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • Domain
    The leucine-zipper is required for dimerization and transcriptional repression.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12CC4 antibody
    • FLJ23741 antibody
    • Fork head related protein like B antibody
    • Forkhead box P1 antibody
    • Forkhead box protein P1 antibody
    • FOX P1 antibody
    • FOXP 1 antibody
    • foxp1 antibody
    • FOXP1_HUMAN antibody
    • Glutamine rich factor 1 antibody
    • hFKH1B antibody
    • HSPC215 antibody
    • MGC12942 antibody
    • MGC88572 antibody
    • MGC99551 antibody
    • QRF 1 antibody
    • QRF1 antibody
    see all

Images

  • Image courtesy of Human Protein Atlas

    ab16645 staining FOXP1 in human small intestine. Paraffin embedded human small intestine tissue was incubated with ab16645 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab16645 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at www.proteinatlas.org

  • ab14404 staining FOXP1 in mouse pancreatic cancer primary cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (1:1), permeabilized with Triton 0.025% in PBS and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in PBS) for 4 hours. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-FOXP1 antibody (ab16645) at 1/5000 dilution

    Lane 1 : Lysate from e12.5 whole mouse embryo FoxP1 +/-
    Lane 2 : Lysate from e12.5 whole mouse embryo FoxP1 -/-

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 75 kDa
    Observed band size: 65,82 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 45 kDa (possible cross reactivity), 50 kDa (possible cross reactivity), 85 kDa (possible cross reactivity)

  • ab16645 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) brain. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/200 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.

References

This product has been referenced in:
  • Harrison DJ  et al. The Effect of Tissue Preparation and Donor Age on Striatal Graft Morphology in the Mouse. Cell Transplant 27:230-244 (2018). Read more (PubMed: 29637815) »
  • Liu AN  et al. Knockdown of LINC01614 inhibits lung adenocarcinoma cell progression by up-regulating miR-217 and down-regulating FOXP1. J Cell Mol Med 22:4034-4044 (2018). Read more (PubMed: 29934982) »
See all 54 Publications for this product

Customer reviews and Q&As

1-10 of 20 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - nuclear (Adipose stromal cells)
Negative control
input / no ab control
Specification
Adipose stromal cells
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: PFA, 1%
Positive control
anti-CEBPB ChIP-seq

Abcam user community

Verified customer

Submitted Jun 02 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (mesenchymal stem cells)
Permeabilization
Yes - Triton 0.25%
Specification
mesenchymal stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 04 2016

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK293T cell line)
Total protein in input
20 µg
Treatment
Calcium Phosphate Transfection of HA-tagged FOXP1
Immuno-precipitation step
Protein G
Specification
HEK293T cell line

Abcam user community

Verified customer

Submitted Nov 18 2015

Application
Flow Cytometry
Sample
Human Cell (Differentiated hNSCs)
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Gating Strategy
Undifferentiated Stem Cells (white)
Specification
Differentiated hNSCs
Preparation
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 15 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (Differentiated hNSCs)
Specification
Differentiated hNSCs
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 16 2015

Abreviews
Application
ChIP
Detection step
Other
Sample
Mouse Cell lysate - nuclear (Spleen)
Specification
Spleen
Negative control
Input
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: HCHO
Positive control
anti-Foxp1

Dr. Xi Chen

Verified customer

Submitted Jul 18 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (artery)
Specification
artery
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (vascular smooth muscle cell)
Loading amount
20 µg
Specification
vascular smooth muscle cell
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 23 2012

Answer

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Read More

Answer

Thank you for your reply and for sending us your data and images. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab16645. I see that you have not performed a permeabilization step in the protocol.  Since this is a nuclear antigen, we would strongly recommend permeabilizing your cells.  This will allow the antibody access to the nucleus. Triton or NP-40 Use 0.1 to 0.2% in PBS for 10 min only. These will also partially dissolve the nuclear membrane and are therefore very suitable for nuclear antigen staining. I would also recommend increasing the blocking to 10% goat serum for 1 hr at RT. Have you run a no-primary control? What dilution have you tried the primary antibody?  If you had tried 1/500, I would recommend diluting more to at least 1/1000 and include 1% BSA in with this. You should also dilute the secondary more to 1/500 or 1/1000 and again include the 1% BSA block. For your washes, you should do 3 washes x 5 mins each.  I couldn't tell how long the washes were from your protocol. Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.  

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1-10 of 20 Abreviews or Q&A

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