Anti-FXYD1/PLM antibody (ab76597)
Key features and details
- Rabbit polyclonal to FXYD1/PLM
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-FXYD1/PLM antibody
See all FXYD1/PLM primary antibodies -
Description
Rabbit polyclonal to FXYD1/PLM -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Macaque monkey -
Immunogen
Synthetic peptide corresponding to Human FXYD1/PLM aa 50 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab88161) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). |
Target
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Function
May have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed. -
Tissue specificity
Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, prostate without endothelial lining, spleen, and testis. -
Sequence similarities
Belongs to the FXYD family. -
Post-translational
modificationsMajor plasma membrane substrate for cAMP-dependent protein kinase (PK-A) and protein kinase C (PK-C) in several different tissues (By similarity). Phosphorylated in response to insulin and adrenergic stimulation. May be phosphorylated by DMPK.
Palmitoylation increases half-life and stability, it is enhanced upon phosphorylation at Ser-88 by PKA. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 476487 Dog
- Entrez Gene: 5348 Human
- Entrez Gene: 56188 Mouse
- Entrez Gene: 58971 Rat
- Omim: 602359 Human
- SwissProt: P56513 Dog
- SwissProt: O00168 Human
- SwissProt: Q9Z239 Mouse
see all -
Alternative names
- FXYD domain containing ion transport regulator 1 antibody
- FXYD domain-containing ion transport regulator 1 antibody
- FXYD1 antibody
see all
Images
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All lanes : Anti-FXYD1/PLM antibody (ab76597) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456)
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 10 kDa
Observed band size: 10 kDa
Exposure time: 5 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab76597 has been referenced in 11 publications.
- Main A et al. Dynamic but discordant alterations in zDHHC5 expression and palmitoylation of its substrates in cardiac pathologies. Front Physiol 13:1023237 (2022). PubMed: 36277202
- Bubb KJ et al. FXYD1 Is Protective Against Vascular Dysfunction. Hypertension 77:2104-2116 (2021). PubMed: 33934624
- Meyer DJ et al. FXYD protein isoforms differentially modulate human Na/K pump function. J Gen Physiol 152:N/A (2020). PubMed: 33231612
- Wang X et al. Comparative analysis of cell lineage differentiation during hepatogenesis in humans and mice at the single-cell transcriptome level. Cell Res 30:1109-1126 (2020). PubMed: 32690901
- Plain F et al. Control of protein palmitoylation by regulating substrate recruitment to a zDHHC-protein acyltransferase. Commun Biol 3:411 (2020). PubMed: 32737405