Anti-Fibrinogen antibody (ab34269)
Key features and details
- Rabbit polyclonal to Fibrinogen
- Suitable for: IHC-P, ICC/IF, Functional Studies, Sandwich ELISA
- Reacts with: Mouse, Rabbit, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Fibrinogen antibody
See all Fibrinogen primary antibodies -
Description
Rabbit polyclonal to Fibrinogen -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, Functional Studies, Sandwich ELISAmore details -
Species reactivity
Reacts with: Mouse, Rabbit, Human
Predicted to work with: Rat -
Immunogen
Full length native protein (purified) corresponding to Human Fibrinogen.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (8) |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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Functional Studies |
Use at an assay dependent concentration.
This antibody has been tested in plasma clotting assays. The plasma clotting time was prolonged (but not completely neutralized) by the antibody. Testing has indicated that these neutralisation assays work only when this antibody has been protein A column purified first. |
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Sandwich ELISA |
1/200.
For sandwich ELISA, use this antibody as Detection at 1/200 dilution with Mouse monoclonal [1F7] to Fibrinopeptide A (ab14790) as Capture. |
Notes |
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IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. This antibody has been tested in plasma clotting assays. The plasma clotting time was prolonged (but not completely neutralized) by the antibody. Testing has indicated that these neutralisation assays work only when this antibody has been protein A column purified first. |
Sandwich ELISA
1/200. For sandwich ELISA, use this antibody as Detection at 1/200 dilution with Mouse monoclonal [1F7] to Fibrinopeptide A (ab14790) as Capture. |
Target
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Function
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Domain
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
Post-translational
modificationsThe alpha chain is not glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylation sites are present in the extracellular medium. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2243 Human
- Entrez Gene: 2244 Human
- Entrez Gene: 2266 Human
- Entrez Gene: 110135 Mouse
- Entrez Gene: 14161 Mouse
- Entrez Gene: 99571 Mouse
- Entrez Gene: 24366 Rat
- Entrez Gene: 24367 Rat
see all -
Alternative names
- FGA antibody
- FGB antibody
- FGG antibody
see all
Images
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Ab34269 staining human liver. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1/50. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Standard Curve for Fibrinogen (Analyte: Fibrinogen protein (Human) (ab84410)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [1F7] to Fibrinopeptide A (ab14790) at 5µg/ml and Detector Antibody Rabbit polyclonal to Fibrinogen (ab34269) at 1/2000.
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ab34269 staining Fibrinogen in Human liver/placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer, pH 6.0 for 20 minutes at 100°C. The primary antibody was diluted 1/2000 and incubated with the sample for 20 minutes at 25°C. An HRP polymer-conjugated mouse anti-rabbit polyclonal IgG was used as the secondary antibody.
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Immunohistochemical analysis of rat heart, staining Fibrinogen with ab34269.
Tissue was fixed with formaldehyde and blocked with 0.25% BSA for 15 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1.5 µg/ml in 1% BSA in TBS) for 20 minutes. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
Protocols
Datasheets and documents
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Datasheet download
References (55)
ab34269 has been referenced in 55 publications.
- Georgiadou A et al. Comparative transcriptomic analysis reveals translationally relevant processes in mouse models of malaria. Elife 11:N/A (2022). PubMed: 35006075
- Pei S et al. Lysophosphatidic Acid Receptor 3 Suppress Neutrophil Extracellular Traps Production and Thrombosis During Sepsis. Front Immunol 13:844781 (2022). PubMed: 35464399
- Ghorbani S et al. Versican promotes T helper 17 cytotoxic inflammation and impedes oligodendrocyte precursor cell remyelination. Nat Commun 13:2445 (2022). PubMed: 35508608
- Sun YE et al. Different Thrombus Histology in a Cancer Patient with Deep Vein Thrombosis and Recurrent Strokes. J Stroke 24:300-302 (2022). PubMed: 35677986
- Zhao C et al. Delayed administration of nafamostat mesylate inhibits thrombin-mediated blood-spinal cord barrier breakdown during acute spinal cord injury in rats. J Neuroinflammation 19:189 (2022). PubMed: 35842640