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Synthetic peptide derived from the N terminal domain of human Filaggrin
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 23rd June 2017. The following lots are still unpurified and still in stock as of 23rd June 2017 - GR309720-5, GR3178284-1, GR309720-6, GR309720-2, GR309720-3, GR309720-4. Lot numbers other than GR309720-5, GR3178284-1, GR309720-6, GR309720-2, GR309720-3, GR309720-4 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
Our Abpromise guarantee covers the use of ab81468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/200 - 1/1000.|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500 - 1/5000. Predicted molecular weight: 435 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
ab81468 staining Filaggrin in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/250) for 2 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
ICC/IF image of ab81468 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81468, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of Human skin sample labeling filaggrin with ab81468. Counterstained with haematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"