Free Glycerol Assay Kit (ab65337)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts
- Sensitivity: 1 µM
Overview
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Product name
Free Glycerol Assay Kit
See all Glycerol kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 1 µM -
Range
1 µM - 10000 µM -
Assay time
0h 40m -
Product overview
Glycerol Assay Kit (ab65337) provides a sensitive, easy assay to measure free glycerol concentration.
In the glycerol assay protocol, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).
This glycerol assay kit can detect 50 pmol-10 nmol (or ~1-10000 µM range) of glycerol.
Glycerol assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K630 Free Glycerol Colorimetric/Fluorometric Assay Kit. K630-100 is the same size as the 100 test size of ab65337.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Glycerol Assay Buffer WM 1 x 25ml Glycerol Enzyme Mix (lyophilized) Green 1 vial Glycerol Probe (in DMSO, anhydrous) Red 1 x 200µl Glycerol Standard (100 mM) Yellow 1 x 200µl -
Research areas
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Relevance
Glycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmetic products, etc. There is broad interest in quantification of glycerol for research and development. -
Alternative names
- Glycerin
- Glycerine
Associated products
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Related Products
Images
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Free Glycerol measured in cell culture lysates showing quantity (nmol) per 1 mln cells.
Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 2-8 fold and measured fluorometrically.
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Free Glycerol measured in biological fluids showing quantity (nmol) per mL of tested sample. Samples were diluted 2-8 fold and measured colorimetrically.
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a. Measurement of free glycerol in pooled human serum (10 μL). b. Measurement of free glycerol in rat liver lysate (500 μg).
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab65337 has been referenced in 16 publications.
- Ishikura S et al. The transcriptional regulator Zfat is essential for maintenance and differentiation of the adipocytes. J Cell Biochem 122:626-638 (2021). PubMed: 33522619
- Huang M et al. Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D. Mol Metab 47:101182 (2021). PubMed: 33549845
- Kim NY et al. Anti-Obesity Effects of Morus alba L. and Aronia melanocarpa in a High-Fat Diet-Induced Obese C57BL/6J Mouse Model. Foods 10:N/A (2021). PubMed: 34441691
- Raineri A et al. 3,5-Dicaffeoylquinic Acid Lowers 3T3-L1 Mitotic Clonal Expansion and Adipocyte Differentiation by Enhancing Heme Oxygenase-1 Expression. Molecules 26:N/A (2021). PubMed: 34443613
- Leiss V et al. Lack of Gai2 proteins in adipocytes attenuates diet-induced obesity. Mol Metab 40:101029 (2020). PubMed: 32480042