Overview

  • Product name
    Free Glycerol Assay Kit
    See all Glycerol kits
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
    Quantitative
  • Sensitivity
    > 1 µM
  • Range
    1 µM - 10000 µM
  • Assay time
    0h 40m
  • Product overview

    Abcam's Glycerol Assay Kit (ab65337) provides a sensitive, easy assay to measure free glycerol concentration in various samples. In the assay, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 50 pmol-10 nmol (or ~1-10000 µM range) of glycerol in various samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmetic products, etc. There is broad interest in quantification of glycerol for research and development.

Properties

Images

  • Functional Studies - Free Glycerol Assay Kit (ab65337)
    Functional Studies - Free Glycerol Assay Kit (ab65337)

    Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Functional Studies - Free Glycerol Assay Kit (ab65337)
    Functional Studies - Free Glycerol Assay Kit (ab65337)

    Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Functional Studies - Free Glycerol Assay Kit (ab65337)
    Functional Studies - Free Glycerol Assay Kit (ab65337)

    Free Glycerol measured in cell culture lysates showing quantity (nmol) per 1 mln cells.

    Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 2-8 fold and measured fluorometrically.

  • Functional Studies - Free Glycerol Assay Kit (ab65337)
    Functional Studies - Free Glycerol Assay Kit (ab65337)

    Free Glycerol measured in biological fluids showing quantity (nmol) per mL of tested sample. Samples were diluted 2-8 fold and measured colorimetrically.

  • Free Glycerol Assay Kit - Functional Studies
    Free Glycerol Assay Kit - Functional Studies

    a. Measurement of free glycerol in pooled human serum (10 μL). b. Measurement of free glycerol in rat liver lysate (500 μg).

Protocols

References

This product has been referenced in:
  • Mukherjee S  et al. Derivation and characterization of a UCP1 reporter human ES cell line. Stem Cell Res 30:12-21 (2018). Read more (PubMed: 29777802) »
  • Honek J  et al. Modulation of age-related insulin sensitivity by VEGF-dependent vascular plasticity in adipose tissues. Proc Natl Acad Sci U S A 111:14906-11 (2014). Functional Studies . Read more (PubMed: 25271320) »

See all 5 Publications for this product

Publishing research using ab65337? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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11 reviews or Q&A

10
Votes: Highest First

Le agradecería que me facilitara información sobre posible interferencia de la cafeína con la sonda fluorescente del kit (quenching) y si conoce algún control positivo lipolítico que no interfiera con las propiedades ...

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Es posible que la cafeína esté interfiriendo a la longitud de onda de lectura de fluorescencia del glicerol (535/587 nm). Como controles alternativos podéis usar isoproterenol (1uM final) o cloroquina.

Ab65337: en el protocolo de su web indica que es un kit con el que podemos detectar glicerol libre por fluorescencia a partir de sobrenadantes celulares. ¿sería posible hacerlo a partir de extractos celulares? ¿cúal ser&iacu...

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Ab65337: Este kit puede usarse a partir de extractos celulares. Para ello, comenzar con un número aproximado de ˜2X10^6 células, suspender el pellet en 500ul (o aproximadamente 4 volúmenes) de assay buffer en hielo usando...

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Can I use the same method of samples preparation in the protocol of Triglyceride Quantification Kit to prepare the samples to use your Free Glycerol Assay Kit to measure intracellular glycerol?

No, I don’t think preparing samples once for both assays is a good idea. For the glycerol assay, you just need cell homogenates, whereas for the triglyceride assay you are actually preparing the lipid extracts from the cells and using them for fu...

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We usually use HEPES lysis buffer to lysate cells. I want to ask can I use your Free Glycerol Assay Kit (ab65337) and Triglyceride Quantification Kit (ab65336) to measure glycerol level and triglyceride level in the cell extracts after I use this kind ...

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Yes, with the Free Glycerol Assay Kit, you can use HEPES for lysis, although the assay buffer provided with the kit is recommended.

With the Triglyceride Quantification Kit, we would recommend using HEPES and NP-40 since the NP-40 is importan...

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This kit is said usable with urine samples. In fact, whereas there is no problem with the standard curve (in buffer), urine samples spiked with glycerol don't give rise to any color even if spiked with glycerol at a concentration greater than the mid p...

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Thank you for your inquiry and your patience with this reply. I will be helping you further as Carolyn is out of the office.
I spoke to our development scientist about this issue and they thought that the urine contains some compound which may be ...

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Hi,



I would like to know the reaction that takes place in your glycerol kit, ab65337.

Which components are a part of it?



Best

Thank you for your inquiry.

The exact description of the components of this kit is proprietary information.

But I am happy to confirm the way this kit works in more detail:

The probe that is used in kit ab65337is a highly ...

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1. We would like to know the testing method or test type ?

Could you send us the instruction for use ?

2. Can this kit test pure glycerol or impure glycerol in Ester and glycerol?



We are looking forward to h...

Read More

Thank you for your inquiry.

Please find the answers to your questions below:

1.)This Glycerol Assay Kit provides a sensitive, easy assay to measure free glycerol concentration in various samples. In the assay, glycerol is enzymatica...

Read More

Hi all,
I need the msds for the free glycerol assay kit (ab65337) mentioned in the subject otherwise I cannot order this item.
Thanks.

Thank you for contacting us.

I am happy to let you know that the Free Glycerol Assay Kitab65337does not contain any hazardous substances.

If you wish we could provide the MSDS for DMSO which would state that it is not a hazardous su...

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Thank you for your email.   Our customer would like to receive a replacement with another lot#.   Please check this and arrange a replacement for our customer.

Thank you for your reply. I have contacted our Distributors Team and they will issue 1 free of charge replacement of ab65337 on your next Abcam order. I checked the lots we have in stock and this will come from a different lot but the same QC b...

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our customer would like to know if the following kits: #AB83390, AB65342, AB83394, AB65337 can be used for cell culture. I see on your datasheet that only the Pyruvate Assay Kit can be used with biological sample such as cell culture, Do you plea...

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All of the kits: ab65337, ab65342, ab83390, ab83394 are suitable for cell and tissue culture supernatants. As well as many other biological fluids like urine, plasma and serum.

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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