Free Glycerol Assay Kit (ab65337)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts
- Sensitivity: 1 µM
Overview
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Product name
Free Glycerol Assay Kit
See all Glycerol kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 1 µM -
Range
1 µM - 10000 µM -
Assay time
0h 40m -
Product overview
Glycerol Assay Kit (ab65337) provides a sensitive, easy assay to measure free glycerol concentration.
In the glycerol assay protocol, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).
This glycerol assay kit can detect 50 pmol-10 nmol (or ~1-10000 µM range) of glycerol.
Glycerol assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K630 Free Glycerol Colorimetric/Fluorometric Assay Kit. K630-100 is the same size as the 100 test size of ab65337.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer V 1 x 25ml Enzyme Mix VI 1 vial Glycerol Standard 1 x 200µl OxiRed Probe 1 x 200µl -
Research areas
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Relevance
Glycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmetic products, etc. There is broad interest in quantification of glycerol for research and development. -
Alternative names
- Glycerin
- Glycerine
Associated products
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Related Products
Images
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Free Glycerol measured in cell culture lysates showing quantity (nmol) per 1 mln cells.
Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 2-8 fold and measured fluorometrically.
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Free Glycerol measured in biological fluids showing quantity (nmol) per mL of tested sample. Samples were diluted 2-8 fold and measured colorimetrically.
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a. Measurement of free glycerol in pooled human serum (10 μL). b. Measurement of free glycerol in rat liver lysate (500 μg).
Datasheets and documents
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SDS download
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Datasheet download
References (29)
ab65337 has been referenced in 29 publications.
- Lechasseur A et al. Glycerol contained in vaping liquids affects the liver and aspects of energy homeostasis in a sex-dependent manner. Physiol Rep 10:e15146 (2022). PubMed: 35075822
- Takeda Y & Dai P Capsaicin directly promotes adipocyte browning in the chemical compound-induced brown adipocytes converted from human dermal fibroblasts. Sci Rep 12:6612 (2022). PubMed: 35459786
- Chiang CH et al. P2X7 Activation Enhances Lipid Accumulation During Adipocytes Differentiation Through Suppressing the Expression of Sirtuin-3, Sirtuin-5, and Browning Genes. Front Pharmacol 13:852858 (2022). PubMed: 35462937
- Wang L et al. Effects of Cigarette Smoke on Adipose and Skeletal Muscle Tissue: In Vivo and In Vitro Studies. Cells 11:N/A (2022). PubMed: 36139468
- Dutta RK et al. Catalase deficiency facilitates the shuttling of free fatty acid to brown adipose tissue through lipolysis mediated by ROS during sustained fasting. Cell Biosci 11:201 (2021). PubMed: 34876210