Anti-G protein beta subunit like/GBL antibody (ab25974)
Key features and details
- Rabbit polyclonal to G protein beta subunit like/GBL
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-G protein beta subunit like/GBL antibody
See all G protein beta subunit like/GBL primary antibodies -
Description
Rabbit polyclonal to G protein beta subunit like/GBL -
Host species
Rabbit -
Specificity
ab25974 recognises GßL (G protein beta protein subunit-like). -
Tested applications
Suitable for: WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human G protein beta subunit like/GBL (C terminal).
(Peptide available asab39766) -
Positive control
- WB: Human brain cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Can be blocked with Human G protein beta subunit like/GBL peptide (ab39766).
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Notes |
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WB
Use a concentration of 1 µg/ml. Can be blocked with Human G protein beta subunit like/GBL peptide (ab39766). |
Target
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Function
Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. -
Tissue specificity
Broadly expressed, with highest levels in skeletal muscle, heart and kidney. -
Sequence similarities
Belongs to the WD repeat LST8 family.
Contains 7 WD repeats. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 64223 Human
- Omim: 612190 Human
- SwissProt: Q9BVC4 Human
- Unigene: 29203 Human
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Alternative names
- G protein beta subunit like antibody
- G protein beta subunit-like antibody
- Gable antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab25974 has been referenced in 1 publication.
- Lin Z et al. Construction of competitive endogenous RNA network reveals regulatory role of long non-coding RNAs in type 2 diabetes mellitus. J Cell Mol Med 21:3204-3213 (2017). PubMed: 28643459