Anti-G-protein coupled receptor 30 antibody (ab137479)
Key features and details
- Rabbit polyclonal to G-protein coupled receptor 30
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-G-protein coupled receptor 30 antibody
See all G-protein coupled receptor 30 primary antibodies -
Description
Rabbit polyclonal to G-protein coupled receptor 30 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 244-336 of Human G-protein coupled receptor 30 (Uniprot: Q99527).
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Positive control
- HepG2 membrane lysate; Human SNU1 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 42 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 42 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for estrogen. -
Tissue specificity
Ubiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Protein has been detected in the cell membrane, endoplasmic reticulum and Golgi apparatus. It is currently unclear whether this is a cell surface or intracellular receptor. - Information by UniProt
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Database links
- Entrez Gene: 2852 Human
- Omim: 601805 Human
- SwissProt: Q99527 Human
- Unigene: 20961 Human
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Alternative names
- CEPR antibody
- Chemoattractant receptor-like 2 antibody
- Chemokine receptor-like 2 antibody
see all
Images
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Anti-G-protein coupled receptor 30 antibody (ab137479) at 1/500 dilution + HepG2 membrane lysate
at 30 µg
Predicted band size: 42 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin embedded Human SNU1 xenograft tissue labelling G-protein coupled receptor 30 with ab137479 antibody at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab137479 has been referenced in 7 publications.
- Leffler KE & Abdel-Rahman AA Restoration of Adiponectin-Connexin43 Signaling Mitigates Myocardial Inflammation and Dysfunction in Diabetic Female Rats. J Cardiovasc Pharmacol 75:259-267 (2020). PubMed: 31868825
- Lappano R et al. The IL1ß-IL1R signaling is involved in the stimulatory effects triggered by hypoxia in breast cancer cells and cancer-associated fibroblasts (CAFs). J Exp Clin Cancer Res 39:153 (2020). PubMed: 32778144
- Vivacqua A et al. Differential MicroRNA Landscape Triggered by Estrogens in Cancer Associated Fibroblasts (CAFs) of Primary and Metastatic Breast Tumors. Cancers (Basel) 11:N/A (2019). PubMed: 30909585
- Santolla MF et al. GPER Mediates a Feedforward FGF2/FGFR1 Paracrine Activation Coupling CAFs to Cancer Cells toward Breast Tumor Progression. Cells 8:N/A (2019). PubMed: 30866584
- Cirillo F et al. AHR and GPER mediate the stimulatory effects induced by 3-methylcholanthrene in breast cancer cells and cancer-associated fibroblasts (CAFs). J Exp Clin Cancer Res 38:335 (2019). PubMed: 31370872
- Deng J et al. MicroRNA-195 inhibits epithelial-mesenchymal transition by targeting G protein-coupled estrogen receptor 1 in endometrial carcinoma. Mol Med Rep 20:4023-4032 (2019). PubMed: 31545414
- Vivacqua A et al. miR-338-3p Is Regulated by Estrogens through GPER in Breast Cancer Cells and Cancer-Associated Fibroblasts (CAFs). Cells 7:N/A (2018). PubMed: 30423928