Anti-GABA A Receptor alpha 2/GABRA2 antibody [N399/19] (ab193311)
Key features and details
- Mouse monoclonal [N399/19] to GABA A Receptor alpha 2/GABRA2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-GABA A Receptor alpha 2/GABRA2 antibody [N399/19]
See all GABA A Receptor alpha 2/GABRA2 primary antibodies -
Description
Mouse monoclonal [N399/19] to GABA A Receptor alpha 2/GABRA2 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: SKNSH cell line.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N399/19 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 52,104 kDa (predicted molecular weight: 51 kDa).
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 52,104 kDa (predicted molecular weight: 51 kDa). |
Target
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Function
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA2 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2555 Human
- Entrez Gene: 14395 Mouse
- Entrez Gene: 289606 Rat
- Omim: 137140 Human
- SwissProt: P47869 Human
- SwissProt: P26048 Mouse
- SwissProt: P23576 Rat
- Unigene: 116250 Human
see all -
Alternative names
- GABA A receptor subunit alpha 2 antibody
- GABA antibody
- GABA(A) receptor subunit alpha 2 antibody
see all
Images
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All lanes : Anti-GABA A Receptor alpha 2/GABRA2 antibody [N399/19] (ab193311) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Human brain tissue lysate - total protein (ab29466)
Lane 4 : Pancreas (Rat) Tissue Lysate
Lane 5 : Human pancreas tissue lysate - total protein (ab29816)
Lane 6 : Pancreas (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Additional bands at: 104 kDa (possible dimer)
Exposure time: 20 minutes -
ICC/IF image of ab193311 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab193311 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Datasheets and documents
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Datasheet download
References (6)
ab193311 has been referenced in 6 publications.
- Le AA et al. Prepubescent female rodents have enhanced hippocampal LTP and learning relative to males, reversing in adulthood as inhibition increases. Nat Neurosci 25:180-190 (2022). PubMed: 35087246
- Zhang W et al. Lactobacillus reuteri normalizes altered fear memory in male Cntnap4 knockout mice. EBioMedicine 86:104323 (2022). PubMed: 36395738
- Wang S et al. Role of GABAAR in the Transition From Acute to Chronic Pain and the Analgesic Effect of Electroacupuncture on Hyperalgesic Priming Model Rats. Front Neurosci 15:691455 (2021). PubMed: 34220444
- Zhang W et al. Effect of Jian-Pi-Zhi-Dong Decoction on the Amino Acid Neurotransmitters in a Rat Model of Tourette Syndrome and Comorbid Anxiety Disorder. Front Psychiatry 11:515 (2020). PubMed: 32581885
- Scott H et al. Maternal antioxidant treatment prevents the adverse effects of prenatal stress on the offspring's brain and behavior. Neurobiol Stress 13:100281 (2020). PubMed: 33344732
- Zhang W et al. Effect of Yi-nao-jie-yu decoction on ?-aminobutyric acid type A receptor in the hippocampus and serum inflammatory factors in a rat model of poststroke anxiety. Neuropsychiatr Dis Treat 12:2827-2837 (2016). WB ; Rat . PubMed: 27843317