Overview

  • Product name

    Anti-GABA B Receptor 1 antibody
    See all GABA B Receptor 1 primary antibodies
  • Description

    Rabbit polyclonal to GABA B Receptor 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Orangutan
  • Immunogen

    Synthetic peptide corresponding to amino acids 892-914 (

    EKENRELEKIIAEKEERVSELRH

    ) of Human GABA B Receptor 1. (sequence is common to isoforms 1A,1B and 1C).

Properties

Applications

Our Abpromise guarantee covers the use of ab90883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/3000. Do not perform antigen retrieval.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by (-)-baclofen, cgp27492 and blocked by phaclofen.
    Isoform 1E function may be to regulate the availability of functional GABA-B-R1A/GABA-B-R2 heterodimers by competing for GABA-B-R2 dimerization. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.
  • Tissue specificity

    Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
    Contains 2 Sushi (CCP/SCR) domains.
  • Domain

    Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 2. The linker region between the transmembrane domain 3 (TM3) and the transmembrane domain 4 (TM4) probably play a role in the specificity for G-protein coupling.
  • Cellular localization

    Secreted and Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Colocalizes with ATF4 in hippocampal neuron dendritic membranes (By similarity). Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • dJ271M21.1.1 antibody
    • dJ271M21.1.2 antibody
    • FLJ92613 antibody
    • GABA-B receptor 1 antibody
    • GABA-B-R1 antibody
    • GABA-BR1 antibody
    • GABAB R1 antibody
    • GABAB subunit 1c antibody
    • GABABR1 antibody
    • GABBR1 3 antibody
    • GABBR1 antibody
    • GABR1_HUMAN antibody
    • Gamma aminobutyric acid (GABA) B receptor 1 antibody
    • Gamma-aminobutyric acid type B receptor subunit 1 antibody
    • Gb1 antibody
    • GPRC3A antibody
    • Seven transmembrane helix receptor antibody
    see all

References

This product has been referenced in:

  • Qiao LN  et al. Contribution of GABAergic modulation in DRGs to electroacupuncture analgesia in incisional neck pain rats. J Pain Res 12:405-416 (2019). Read more (PubMed: 30705606) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

I have had a chance to review each of the product which you have listed as well as our other products against the same targets. I would like to offer some recommendations.

Anti GABA A receptor alpha 1 subunit antibody: ab33299 (https://www.abcam.com/GABA-A-Receptor-alpha-1-antibody-ab33299.html) and ab86320 (https://www.abcam.com/GABA-A-Receptor-alpha-1-antibody-ab86320.html) will both recognize the extracellular region of this protein in human tissue. Each will work as well as the other with the only difference being that ab86320 was created fromHuman GABA A Receptor alpha 1 andhas 100% homology, while ab33299 is created fromRat GABA A Receptor alpha 1with 93% homology.

Anti GABA A receptor beta 2 subunit antibody: Ab42598 (https://www.abcam.com/GABA-A-Receptor-beta-2-antibody-ab42598.html)is raised against the extracellular region of this protein and will react with human tissues.

Anti GABA B receptor 1 antibody: ab90883 (https://www.abcam.com/GABA-B-Receptor-1-antibody-ab90883.html) recognizes a cytoplasmic region of this protein and will require permeabilzation of the cells to be effective. Alternately ab55051 (https://www.abcam.com/GABA-B-Receptor-1-antibody-ab55051.html) recognizes the extracellular region of this protein. This will also work with human tissues.

None of our antibodies against these targets have been tested in flow cytometry. As such I may offer you enrollment in our 100% Abreview Testing Discount. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

I hope this information is helpful to you. Please let me know if you have any questions or would like to take advantage of our testing discount with these products.


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