Recombinant Anti-GABA Transporter 1 / GAT 1 antibody [EPR12999(2)] (ab185205)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12999(2)] to GABA Transporter 1 / GAT 1
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
-
Product name
Anti-GABA Transporter 1 / GAT 1 antibody [EPR12999(2)]
See all GABA Transporter 1 / GAT 1 primary antibodies -
Description
Rabbit monoclonal [EPR12999(2)] to GABA Transporter 1 / GAT 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Mouse and rat brain lysate
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12999(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
|
Notes |
---|
WB
1/10000 - 1/50000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). |
Target
-
Function
Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. -
Sequence similarities
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily. -
Domain
The PDZ domain-binding motif is involved in the interaction with MPP5. -
Cellular localization
Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons. - Information by UniProt
-
Database links
- Entrez Gene: 6529 Human
- Entrez Gene: 232333 Mouse
- Entrez Gene: 79212 Rat
- Omim: 137165 Human
- SwissProt: P30531 Human
- SwissProt: P31648 Mouse
- SwissProt: P23978 Rat
- Unigene: 443874 Human
see all -
Alternative names
- GABATHG antibody
- GABATR antibody
- GABT 1 antibody
see all
Images
-
All lanes : Anti-GABA Transporter 1 / GAT 1 antibody [EPR12999(2)] (ab185205) at 1/20000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 67 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (1)
ab185205 has been referenced in 1 publication.
- Zhou Q et al. MicroRNA 322-5p reduced neuronal inflammation via the TLR4/TRAF6/NF-κB axis in a rat epilepsy model. Open Med (Wars) 17:907-914 (2022). PubMed: 35647304