Goat polyclonal to GAD67/GAD1 (ab80589)
Key features and details
- Goat polyclonal to GAD67/GAD1
- Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Goat polyclonal to GAD67/GAD1
See all GAD67/GAD1 primary antibodies -
Host species
Goat -
Specificity
ab80589 is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2 (isoform GAD65). -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Cat, Dog, Pig, Chimpanzee, Orangutan -
Immunogen
Synthetic peptide corresponding to Human GAD1/GAD67 aa 500 to the C-terminus (internal sequence) (Cysteine residue). NP_000808.2.
Database link: Q99259-1 -
Positive control
- WB: Human brain (cerebral cortex) lysate in RIPA buffer. Flow Cyt: A431 cells; ICC/IF: A431 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab80589 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 10 µg/ml.
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ICC/IF |
Use a concentration of 10 µg/ml.
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 67 kDa.
1 hour primary incubation is recommended for this product. |
Notes |
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Flow Cyt
Use a concentration of 10 µg/ml. |
ICC/IF
Use a concentration of 10 µg/ml. |
IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 67 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
Catalyzes the production of GABA. -
Tissue specificity
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. -
Involvement in disease
Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture. -
Sequence similarities
Belongs to the group II decarboxylase family. - Information by UniProt
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Database links
- Entrez Gene: 493699 Cat
- Entrez Gene: 468557 Chimpanzee
- Entrez Gene: 517552 Cow
- Entrez Gene: 478794 Dog
- Entrez Gene: 2571 Human
- Entrez Gene: 100173252 Orangutan
- Entrez Gene: 396928 Pig
- Omim: 605363 Human
see all -
Alternative names
- 67 kDa glutamic acid decarboxylase antibody
- CPSQ1 antibody
- DCE1 antibody
see all
Images
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Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Goat polyclonal to GAD67/GAD1 (ab80589) at 0.5 µg/ml + Human brain (cerebral cortex) lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 67 kDa
Observed band size: 67 kDa -
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing plasma membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
Datasheets and documents
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Datasheet download
References (5)
ab80589 has been referenced in 5 publications.
- Yao H et al. Methadone interrupts neural growth and function in human cortical organoids. Stem Cell Res 49:102065 (2020). PubMed: 33137567
- Ellis JK et al. Ferret brain possesses young interneuron collections equivalent to human postnatal migratory streams. J Comp Neurol N/A:N/A (2019). PubMed: 31050805
- Corbridge SM et al. JC virus infection of meningeal and choroid plexus cells in patients with progressive multifocal leukoencephalopathy. J Neurovirol N/A:N/A (2019). PubMed: 31025264
- Zhuang QX et al. Regularizing firing patterns of rat subthalamic neurons ameliorates parkinsonian motor deficits. J Clin Invest 128:5413-5427 (2018). PubMed: 30226827
- Taylor SR et al. GABAergic and glutamatergic efferents of the mouse ventral tegmental area. J Comp Neurol 522:3308-34 (2014). IHC-FrFl ; Mouse . PubMed: 24715505