• Product name
    Anti-GATA4 antibody [EPR4768]
    See all GATA4 primary antibodies
  • Description
    Rabbit monoclonal [EPR4768] to GATA4
  • Host species
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human GATA4 aa 1-100 (N terminal).
    Database link: P43694

  • Positive control
    • HepG2, Caco-2 and HeLa whole cell lysate (ab150035); HepG2 cells.
  • General notes

    This antibody was developed as part of a collaboration between Dartmouth College and the lab of Sergei Tevosian.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab134057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
Flow Cyt 1/600.

For unpurified use at 1/1200.





ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


ICC/IF 1/150.

For unpurified use at 1/100 - 1/250.

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
    • Involvement in disease
      Atrial septal defect 2
      Ventricular septal defect 1
      Tetralogy of Fallot
      Atrioventricular septal defect 4
      Testicular anomalies with or without congenital heart disease
      GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Sequence similarities
      Contains 2 GATA-type zinc fingers.
    • Post-translational
      Methylation at Lys-300 attenuates transcriptional activity.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • ASD2 antibody
      • GATA 4 antibody
      • GATA binding protein 4 antibody
      • GATA-binding factor 4 antibody
      • gata4 antibody
      • GATA4_HUMAN antibody
      • MGC126629 antibody
      • Transcription factor GATA 4 antibody
      • Transcription factor GATA-4 antibody
      • Transcription factor GATA4 antibody
      • VSD1 antibody
      see all


    • ab134057 staining GATA4 in the PC-3 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150).  An Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

    • Overlay histogram showing Caco-2 cells stained with ab134057 (red line) at 1/600 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

    • Anti-GATA4 antibody [EPR4768] (ab134057) at 1/2000 dilution + Mouse spleen lysate at 10 µg

      Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size: 54 kDa

    • Anti-GATA4 antibody [EPR4768] (ab134057) at 1/2000 dilution + HepG2 lysate at 10 µg

      Goat Anti-Rabbit IgG, (H+L), HRP-conjugated

      Predicted band size: 54 kDa

    • Overlay histogram showing HeLa cells stained with ab134057, unpurified (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134057, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • All lanes : Anti-GATA4 antibody [EPR4768] (ab134057) at 1/1000 dilution (unpurified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : Caco-2 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 54 kDa

    • Immunofluorescent analysis of HepG2 cells labelling GATA4 with ab134057, unpuified, at 1/100 dilution.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:
    • Wu L  et al. Bone morphogenetic protein 4 promotes the differentiation of Tbx18-positive epicardial progenitor cells to pacemaker-like cells. Exp Ther Med 17:2648-2656 (2019). Read more (PubMed: 30906456) »
    • Hernandez-Cortes D  et al. Epigenetic modulation of Nrf2 and Ogg1 gene expression in testicular germ cells by methyl parathion exposure. Toxicol Appl Pharmacol 346:19-27 (2018). ICC/IF ; Mouse . Read more (PubMed: 29540303) »
    See all 10 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
    Human Cell (Human pluripotent stem cell derived cardiomyocyte)
    Human pluripotent stem cell derived cardiomyocyte
    Yes - saponin

    Abcam user community

    Verified customer

    Submitted Sep 19 2014

    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (10% bottom gel, 4% top gel)
    Sheep Tissue lysate - whole (cardiac muscle - left ventricle)
    cardiac muscle - left ventricle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Adrienne Wilburn

    Verified customer

    Submitted Jul 14 2014


    Thanks your for your email.
    I am sorry to hear that the results does not improve. These antibodies has been positively tested so we are unsure about the cause of no bands.
    I can provide you replacement antibodies if you are interested in using different vials but same lot. In case you want to try a completely different antibody or want a refund for your purchase, I can also help you with that.
    Could let me know how you would like to proceed?

    Read More


    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.
    I have read the details you have kindly provided and have following further suggestions for improvements;
    It is always good to try the antibodies overnight when they fails to show results after couple of hours incubation. May I suggest you to try this.
    MCF7 is not totally negative in GATA4 it however have low expression of GATA4 (http://mend.endojournals.org/content/25/7/1126.full.pdf) (http://cancerres.aacrjournals.org/content/66/3/1384.full) so this might not be a good negative control.
    There is very faint band in G4 lysates near to band of interest. Is this a purified protein lysates?
    Thank you very much for your cooperation. Please let me know if these antibodies fails again I will be happy to process the refund.

    Read More


    Thank you for contacting us.
    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
    I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
    I look forward to receiving your reply.

    Read More


    Thank you for contacting us.

    ab134057 and ab86371 have not been purified and therefore, their concentration has not been determined.

    However we predict that ab134057, which is a tissue culture supernatant, will have a concentration in the region of 1-3 mg/ml.

    We predict that ab86371, which is an ascites, will have a concentration in the region of 5-10mg/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

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