Anti-GFAP antibody [GA-5] - BSA and Azide free (ab212398)
Key features and details
- Mouse monoclonal [GA-5] to GFAP - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-GFAP antibody [GA-5] - BSA and Azide free
See all GFAP primary antibodies -
Description
Mouse monoclonal [GA-5] to GFAP - BSA and Azide free -
Host species
Mouse -
Specificity
ab212398 shows no cross-reactivity with other intermediate filament proteins. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Pig GFAP. GFAP isolated from Pig spinal cord
Database link: F1RR02 -
Positive control
- Human cerebellum whole cell lysate, Human cerebellum tissue, Brain or Astrocytoma.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor Concentrate -
Clonality
Monoclonal -
Clone number
GA-5 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 49 kDa.
|
Notes |
---|
IHC-P
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 49 kDa. |
Target
-
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
Tissue specificity
Expressed in cells lacking fibronectin. -
Involvement in disease
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylated by PKN1. -
Cellular localization
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
-
Database links
- Entrez Gene: 2670 Human
- Omim: 137780 Human
- SwissProt: P14136 Human
- Unigene: 514227 Human
-
Alternative names
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
Images
Protocols
Datasheets and documents
-
Datasheet download
References (2)
ab212398 has been referenced in 2 publications.
- Léger-Charnay E et al. Retinal cholesterol metabolism is perturbated in response to experimental glaucoma in the rat. PLoS One 17:e0264787 (2022). PubMed: 35275950
- Li Y et al. A novel model of persistent retinal neovascularization for the development of sustained anti-VEGF therapies. Exp Eye Res 174:98-106 (2018). IHC-P . PubMed: 29852133