Overview

  • Product name

    Anti-GFM1 antibody [EPR12263-33]
    See all GFM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12263-33] to GFM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human GFM1. The exact sequence is proprietary.
    Database link: Q96RP9

  • Positive control

    • HeLa, NCI-H460, U87-MG and 293T cell lysates. HeLa cells. Immunoprecipitation pellet from HeLa whole cell lysate (ab150035).
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab173529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 83 kDa.
IP 1/10 - 1/100.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis.
    • Pathway

      Protein biosynthesis; polypeptide chain elongation.
    • Involvement in disease

      Defects in GFM1 are the cause of combined oxidative phosphorylation deficiency type 1 (COXPD1) [MIM:609060]. It leads to early fatal progressive hepatoencephalopathy.
    • Sequence similarities

      Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
    • Cellular localization

      Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • COXPD1 antibody
      • EF-Gmt antibody
      • EFG antibody
      • EFG1 antibody
      • EFGM antibody
      • EFGM_HUMAN antibody
      • EGF1 antibody
      • Elongation factor G 1 antibody
      • Elongation factor G 1 mitochondrial antibody
      • Elongation factor G antibody
      • Elongation factor G1 antibody
      • FLJ12662 antibody
      • FLJ13632 antibody
      • FLJ20773 antibody
      • G elongation factor mitochondrial 1 antibody
      • G translation elongation factor mitochondrial antibody
      • GFM 1 antibody
      • GFM antibody
      • gfm1 antibody
      • hEFG1 antibody
      • mEF G 1 antibody
      • mEF-G 1 antibody
      • mEFG 1 antibody
      • mitochondrial antibody
      • Mitochondrial elongation factor G1 antibody
      see all

    Images

    • All lanes : Anti-GFM1 antibody [EPR12263-33] (ab173529) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : NCI-H460 cell lysates
      Lane 3 : U87-MG cell lysates
      Lane 4 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 83 kDa

    • Immunofluorescence analysis of HeLa cells labeling GFM1 with ab173529 at a 1/50 dilution.

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate: ab173529 at a 1/10 dilution was used for immunoprecipitate GFM1.

    References

    This product has been referenced in:

    • Yokokawa T  et al. Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle. Physiol Rep 6:e13893 (2018). Read more (PubMed: 30369085) »
    • Brito S  et al. Long-term survival in a child with severe encephalopathy, multiple respiratory chain deficiency and GFM1 mutations. Front Genet 6:102 (2015). WB . Read more (PubMed: 25852744) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Hippocampus)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    5 µg
    Specification
    Hippocampus
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jan 15 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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