• Product name

    Anti-GFM1 antibody [EPR12263]
    See all GFM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12263] to GFM1
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human GFM1. The exact sequence is proprietary.
    Database link: Q96RP9

  • Positive control

    • HeLa, NCIH460, U87 MG and 293T cell lysates; Human cervical carcinoma tissue and Human kidney tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab171945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 83 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis.
    • Pathway

      Protein biosynthesis; polypeptide chain elongation.
    • Involvement in disease

      Defects in GFM1 are the cause of combined oxidative phosphorylation deficiency type 1 (COXPD1) [MIM:609060]. It leads to early fatal progressive hepatoencephalopathy.
    • Sequence similarities

      Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • COXPD1 antibody
      • EF-Gmt antibody
      • EFG antibody
      • EFG1 antibody
      • EFGM antibody
      • EFGM_HUMAN antibody
      • EGF1 antibody
      • Elongation factor G 1 antibody
      • Elongation factor G 1 mitochondrial antibody
      • Elongation factor G antibody
      • Elongation factor G1 antibody
      • FLJ12662 antibody
      • FLJ13632 antibody
      • FLJ20773 antibody
      • G elongation factor mitochondrial 1 antibody
      • G translation elongation factor mitochondrial antibody
      • GFM 1 antibody
      • GFM antibody
      • gfm1 antibody
      • hEFG1 antibody
      • mEF G 1 antibody
      • mEF-G 1 antibody
      • mEFG 1 antibody
      • mitochondrial antibody
      • Mitochondrial elongation factor G1 antibody
      see all


    • All lanes : Anti-GFM1 antibody [EPR12263] (ab171945) at 1/10000 dilution

      Lane 1 : HeLa cell line lysate
      Lane 2 : NCI 460 cell line lysate
      Lane 3 : U87 MG cell line lysate
      Lane 4 : 293T cell line lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 83 kDa

    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling GFM1 with ab171945 at 1/50

    • Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling GFM1 with ab171945 at 1/50.


    This product has been referenced in:

    • Simon MT  et al. Activation of a cryptic splice site in the mitochondrial elongation factor GFM1 causes combined OXPHOS deficiency. Mitochondrion 34:84-90 (2017). Read more (PubMed: 28216230) »
    See 1 Publication for this product

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