Anti-GGA2 antibody (ab10552)
Key features and details
- Rabbit polyclonal to GGA2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GGA2 antibody
See all GGA2 primary antibodies -
Description
Rabbit polyclonal to GGA2 -
Host species
Rabbit -
Specificity
This antibody recognises two bands by Western at 75 and 37kD. This corresponds to results seen in Hela extracts in Dell'Angelica et al. 2000. The lower 37kD band is expected to correspond to the isoform described in NP_619581. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide:
LSMYRRPGQAPPDQEAL
conjugated to KLH, corresponding to amino acids 249-265 of Human GGA2. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Detects a band of approximately 75, 37 kDa (predicted molecular weight: 67 kDa).
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Notes |
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WB
1/500. Detects a band of approximately 75, 37 kDa (predicted molecular weight: 67 kDa). |
Target
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Function
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain. -
Domain
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function. -
Post-translational
modificationsUbiquitinated. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 23062 Human
- Entrez Gene: 74105 Mouse
- Omim: 606005 Human
- SwissProt: Q9UJY4 Human
- SwissProt: Q6P5E6 Mouse
- Unigene: 460336 Human
- Unigene: 29619 Mouse
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Alternative names
- 1200007E24Rik antibody
- ADP ribosylation factor binding protein 2 antibody
- ADP ribosylation factor binding protein GGA 2 antibody
see all
Images
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Western blot using ab10552 at 1/500. Lysates at 5µg per lane. Secondary antibody anti-rabbit HRP ab6721 at 1/5000.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: 293Western blot using ab10552 at 1/500. Lysates at 5µg per lane. Secondary ab6721 at 1/5000. Lane 1: Hela nuclear Lane 2: Hela Lane 3: A431 Lane 4: Jurkat Lane 5: 293
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab10552 has been referenced in 3 publications.
- Duffney LJ et al. Epigenetics and autism spectrum disorder: A report of an autism case with mutation in H1 linker histone HIST1H1E and literature review. Am J Med Genet B Neuropsychiatr Genet 177:426-433 (2018). PubMed: 29704315
- Herskowitz JH et al. GGA1-mediated endocytic traffic of LR11/SorLA alters APP intracellular distribution and amyloid-ß production. Mol Biol Cell 23:2645-57 (2012). WB, ICC/IF ; Human . PubMed: 22621900
- Lefrançois S & McCormick PJ The Arf GEF GBF1 is required for GGA recruitment to Golgi membranes. Traffic 8:1440-51 (2007). ICC/IF ; African green monkey . PubMed: 17666033