Anti-GLP-1 antibody [11E2] (ab23468)
Key features and details
- Mouse monoclonal [11E2] to GLP-1
- Suitable for: ELISA, Sandwich ELISA, ICC/IF
- Reacts with: Rat
- Isotype: IgG2a
Overview
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Product name
Anti-GLP-1 antibody [11E2]
See all GLP-1 primary antibodies -
Description
Mouse monoclonal [11E2] to GLP-1 -
Host species
Mouse -
Specificity
This antibody reacts with all forms of GLP-1, including precursor.
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Tested applications
Suitable for: ELISA, Sandwich ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Guinea pig, Cow -
Immunogen
Synthetic peptide corresponding to Human GLP-1 aa 50-150. The immunogen sequence corresponds to Glucagon-like peptide 1(7-36), one of the chains formed when cleaved. Immunogen was coupled to a carrier and adsorbed onto aluminum hydroxide gel.
Database link: P01275 -
Epitope
This antibody reacts with a mid-molecular epitope of GLP 1 (Glucagon-like peptide 1(7-36)). -
Positive control
- ICC/IF: PC12 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading...
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Purification notes
Protein A/G purified -
Clonality
Monoclonal -
Clone number
11E2 -
Myeloma
x63-Ag8.653 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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Sandwich ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
Sandwich ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
GLP-1 is a potent stimulator of glucose-dependent insulin release. Play important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Have growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferaton. Inhibits beta cell apoptosis.
GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.
Oxyntomodulin significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.
Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life. -
Tissue specificity
Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain. -
Sequence similarities
Belongs to the glucagon family. -
Post-translational
modificationsProglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 280802 Cow
- Entrez Gene: 100135526 Guinea pig
- Entrez Gene: 24952 Rat
- SwissProt: P01272 Cow
- SwissProt: P05110 Guinea pig
- SwissProt: P06883 Rat
- Unigene: 54383 Rat
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Alternative names
- GCG antibody
- Glicentin related polypeptide antibody
- glicentin-related polypeptide antibody
see all
Images
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ELISA graph displaying different concentrations of ab23468 used to detect 1 µg/mL of the GLP-1 (GLP-1 (1-36)amide, GLP-1 (9-36)amide, GLP-1 (7-36)amide, and GLP-1 (7-37)) coated directly in the well.
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Sandwich ELISA graph showing GLP-1 (1-36)amide, GLP-1 (9-36)amide, and GLP-1 (7-36)amide detection using ab26278 as catching antibody and biotinylated ab23468 detection antibody.
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ICC/IF image of ab23468 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23468, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab23468 has been referenced in 13 publications.
- Guo S et al. Matrine, as a CaSR agonist promotes intestinal GLP-1 secretion and improves insulin resistance in diabetes mellitus. Phytomedicine 84:153507 (2021). PubMed: 33636577
- Lalitha N et al. Anti-hyperglycemic activity of myricetin, through inhibition of DPP-4 and enhanced GLP-1 levels, is attenuated by co-ingestion with lectin-rich protein. PLoS One 15:e0231543 (2020). PubMed: 32282828
- Calderon G et al. Ileo-colonic delivery of conjugated bile acids improves glucose homeostasis via colonic GLP-1-producing enteroendocrine cells in human obesity and diabetes. EBioMedicine 55:102759 (2020). PubMed: 32344198
- Wei M et al. A dysregulated bile acid-gut microbiota axis contributes to obesity susceptibility. EBioMedicine 55:102766 (2020). PubMed: 32408110
- Rattanaamnuaychai P et al. Direct suppression of human islet dedifferentiation, progenitor genes, but not epithelial to mesenchymal transition by liraglutide. Heliyon 6:e04951 (2020). PubMed: 32995630