Anti-GLS2 antibody (ab113509)
Key features and details
- Rabbit polyclonal to GLS2
- Suitable for: ICC, IHC-Fr, WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-GLS2 antibody
See all GLS2 primary antibodies -
Description
Rabbit polyclonal to GLS2 -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 100% homology with GLS1. Cross-reactivity with this protein has not been confirmed experimentally.
WB has been tested in rat only.
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Tested applications
Suitable for: ICC, IHC-Fr, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human GLS2 (internal sequence).
(Peptide available asab169954) -
Positive control
- WB: Rat kidney lysate. IHC-F: Rat brain tissue. ICC: Rat liver cells. IHC-P: Mouse brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 20 µg/ml.
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IHC-Fr |
Use at an assay dependent concentration.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 66 kDa.
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IHC-P | (1) |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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ICC
Use a concentration of 20 µg/ml. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 66 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. -
Tissue specificity
Highly expressed in liver. Expressed in brain and pancreas. Not observed in heart, placenta, lung, skeletal muscle and kidney. Expression is significantly reduced in hepatocellular carcinomas. -
Sequence similarities
Belongs to the glutaminase family.
Contains 2 ANK repeats. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 216456 Mouse
- Entrez Gene: 192268 Rat
- SwissProt: Q571F8 Mouse
- SwissProt: P28492 Rat
- Unigene: 30102 Mouse
- Unigene: 447148 Mouse
- Unigene: 10202 Rat
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Alternative names
- breast cell glutaminase antibody
- GA antibody
- GLS antibody
see all
Images
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Lane 1 : Anti-GLS2 antibody (ab113509) at 0.5 µg/ml
Lane 2 : Anti-GLS2 antibody (ab113509) at 1 µg/ml
All lanes : GLS2 in Rat Kidney Lysate
Predicted band size: 66 kDa -
Ab113509 IHC-FoFr on rat brain tissue sections. The antibody was used at 1/1000 in the free floating technique for 18 hours at 20°C. There was no blocking step.
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Immunocytochemical analysis of rat liver cells labelling GLS2 with ab113509 at 20 µg/mL.
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Immunohistochemistry of GLS2 in mouse brain tissue with ab113509 at 20 µg/ml (green), followed by DAPI (blue) and phylloidin (red) staining.
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Immunohistochemical analysis of GLS2 in mouse brain with ab113509 at 5 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab113509 has been referenced in 26 publications.
- Franco-García A et al. Molecular Mechanisms Underlying the Retrieval and Extinction of Morphine Withdrawal-Associated Memories in the Basolateral Amygdala and Dentate Gyrus. Biomedicines 10:N/A (2022). PubMed: 35327388
- Shi Z et al. Identification and Validation of a Novel Ferroptotic Prognostic Genes-Based Signature of Clear Cell Renal Cell Carcinoma. Cancers (Basel) 14:N/A (2022). PubMed: 36230613
- Asaba A et al. Prelimbic cortex responds to male ultrasonic vocalizations in the presence of a male pheromone in female mice. Front Neural Circuits 16:956201 (2022). PubMed: 36247727
- Xiong J et al. Glutamine Produces Ammonium to Tune Lysosomal pH and Regulate Lysosomal Function. Cells 12:N/A (2022). PubMed: 36611873
- Guerrero-Bautista R et al. Distinct Regulation of Dopamine D3 Receptor in the Basolateral Amygdala and Dentate Gyrus during the Reinstatement of Cocaine CPP Induced by Drug Priming and Social Stress. Int J Mol Sci 22:N/A (2021). PubMed: 33803578