Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637)
Key features and details
- Rabbit polyclonal to GM130 - Drosophila Golgi/Cis-Golgi Marker
- Suitable for: ICC/IF, WB
- Reacts with: Human, Drosophila melanogaster
- Isotype: IgG
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Overview
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Product name
Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker
See all GM130 primary antibodies -
Description
Rabbit polyclonal to GM130 - Drosophila Golgi/Cis-Golgi Marker -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, Drosophila melanogaster -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Fruit fly (Drosophila melanogaster) GM130.
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Positive control
- Drosophila S2 Cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab30637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (2) |
Use a concentration of 1 µg/ml.
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WB | (1) |
Use a concentration of 0.1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 0.1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa). |
Target
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Function
Golgi auto-antigen; probably involved in maintaining cis-Golgi structure. -
Sequence similarities
Belongs to the GOLGA2 family. -
Domain
Extended rod-like protein with coiled-coil domains. -
Cellular localization
Golgi apparatus > Golgi stack membrane. - Information by UniProt
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Database links
- Entrez Gene: 2801 Human
- Omim: 602580 Human
- SwissProt: Q08379 Human
- Unigene: 155827 Human
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Alternative names
- 130 kDa cis Golgi matrix protein antibody
- 130 kDa cis-Golgi matrix protein antibody
- Cis golgi matrix protein GM130 antibody
see all
Images
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The image shows staining of Drosophila S2 cells using ab30637 to cis-Golgi/Golgi marker, GM130. For comparison, S2 cells were stained with an antibody to the trans-Golgi marker, GCC88. Gm130 stained the cis-Golgi region and appears to be a superior Golgi marker to GCC88.
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All lanes : Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 0.1 µg/ml
Lane 1 : Drosophila melanogaster S2 cell extract
Lane 2 : Drosophila melanogaster S2 cell extract + specific siRNA
Predicted band size: 95 kDa
Observed band size: 95 kDa -
ab30637 at a 1/100 dilution staining Golgi/Cis-Golgi in Drosophila S2R+ cells by Immunocytochemistry/ Immunofluorescence.
Expression of recombinant wild type pgant3 (pIB-pgant3-V5), pgant3m1 (pIB-m1-V5), and pgant3m2 (pIB-m2-V5) in Drosophila S2R+ cells. Cells were then stained for V5 and the Golgi marker GM130 revealing Golgi localization of wild type pgant3 and pgant3m1. -
All lanes : Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 1 µg/ml
Lane 1 : Schneider L2 whole cell lysate (ab14893)
Lane 2 : Schneider L2 whole cell lysate (ab14893) with D. melanogaster GM130 peptide (ab32335) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 95 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (79)
ab30637 has been referenced in 79 publications.
- Wang C et al. Bioinspired therapeutic platform based on extracellular vesicles for prevention of arterial wall remodeling in hypertension. Bioact Mater 8:494-504 (2022). PubMed: 34541415
- Wang R et al. ESCRT dysfunction compromises endoplasmic reticulum maturation and autophagosome biogenesis in Drosophila. Curr Biol 32:1262-1274.e4 (2022). PubMed: 35134326
- Ciesielski HM et al. Erebosis, a new cell death mechanism during homeostatic turnover of gut enterocytes. PLoS Biol 20:e3001586 (2022). PubMed: 35468130
- Tanasic D et al. Myosin V facilitates polarised E-cadherin secretion. Traffic 23:374-390 (2022). PubMed: 35575181
- Rahman A et al. GMAP is an Atg8a-interacting protein that regulates Golgi turnover in Drosophila. Cell Rep 39:110903 (2022). PubMed: 35649355