Anti-GNPAT/DAPAT antibody (ab75060)
Key features and details
- Rabbit polyclonal to GNPAT/DAPAT
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GNPAT/DAPAT antibody -
Description
Rabbit polyclonal to GNPAT/DAPAT -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab75060 was purified using an epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 77 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 77 kDa. |
Target
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Pathway
Membrane lipid metabolism; glycerophospholipid metabolism. -
Involvement in disease
Defects in GNPAT are the cause of rhizomelic chondrodysplasia punctata type 2 (RCDP2) [MIM:222765]. RDCP2 is characterized by rhizomelic shortening of femur and humerus, vertebral disorders, cataract, cutaneous lesions and severe mental retardation. -
Sequence similarities
Belongs to the GPAT/DAPAT family. -
Domain
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. -
Cellular localization
Peroxisome membrane. Exclusively localized to the lumenal side of the peroxisomal membrane. - Information by UniProt
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Database links
- Entrez Gene: 8443 Human
- Entrez Gene: 14712 Mouse
- Omim: 602744 Human
- SwissProt: O15228 Human
- SwissProt: P98192 Mouse
- Unigene: 498028 Human
- Unigene: 29114 Mouse
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Alternative names
- Acyl CoA dihydroxyacetonephosphateacyltransferase antibody
- Acyl-CoA:dihydroxyacetonephosphateacyltransferase antibody
- DAP AT antibody
see all
Images
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All lanes : Anti-GNPAT/DAPAT antibody (ab75060) at 1/500 dilution
Lane 1 : extracts from HT-29 cells
Lane 2 : extracts from RAW264.7cells
Lane 3 : extracts from HT-29 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab75060 has been referenced in 3 publications.
- Hossain MS et al. Plasmalogens, the Vinyl Ether-Linked Glycerophospholipids, Enhance Learning and Memory by Regulating Brain-Derived Neurotrophic Factor. Front Cell Dev Biol 10:828282 (2022). PubMed: 35223852
- Hossain MS et al. Reduction of Ether-Type Glycerophospholipids, Plasmalogens, by NF-?B Signal Leading to Microglial Activation. J Neurosci 37:4074-4092 (2017). PubMed: 28292831
- Hossain MS et al. Neuronal Orphan G-Protein Coupled Receptor Proteins Mediate Plasmalogens-Induced Activation of ERK and Akt Signaling. PLoS One 11:e0150846 (2016). WB . PubMed: 26934370