Recombinant HRP Anti-GPCR LGR6 antibody [EPR6874] (ab205760)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR6874] to GPCR LGR6
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-GPCR LGR6 antibody [EPR6874]
See all GPCR LGR6 primary antibodies -
Description
HRP Rabbit monoclonal [EPR6874] to GPCR LGR6 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human Adrenal Gland tissue lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6874 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
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Notes |
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WB
1/2000. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa). |
Target
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Function
Orphan receptor. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Contains 15 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 59352 Human
- Entrez Gene: 329252 Mouse
- Entrez Gene: 498233 Rat
- Omim: 606653 Human
- SwissProt: Q9HBX8 Human
- SwissProt: Q3UVD5 Mouse
- Unigene: 497402 Human
- Unigene: 279594 Mouse
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Alternative names
- FLJ14471 antibody
- Gonadotropin receptor antibody
- GPCR antibody
see all
Images
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HRP Anti-GPCR LGR6 antibody [EPR6874] (ab205760) at 1/2000 dilution + Adrenal (Human) Whole Cell Lysate - adult normal tissue at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa
Observed band size: 104 kDa
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205760 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab205760 has been referenced in 1 publication.
- Wang F et al. Downregulation of Lgr6 inhibits proliferation and invasion and increases apoptosis in human colorectal cancer. Int J Mol Med 42:625-632 (2018). PubMed: 29693156