Product nameAnti-GRB2 antibody
See all GRB2 primary antibodies
DescriptionRabbit polyclonal to GRB2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Macaque monkey, Orangutan
- Recombinant Human GRB2 protein (ab85917) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: Hela; Hek293; A431; A549; Ramos; HepG2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab79876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionAdapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
Sequence similaritiesBelongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
DomainThe SH3 domains mediate interaction with RALGPS1 and SHB.
Cellular localizationGolgi apparatus.
- Information by UniProt
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
All lanes : Anti-GRB2 antibody (ab79876) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 5 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Additional bands at: 14 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
GRB2 contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted.
ab79876 has not yet been referenced specifically in any publications.