Anti-GRF2 antibody (ab251683)
Key features and details
- Rabbit polyclonal to GRF2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-GRF2 antibody -
Description
Rabbit polyclonal to GRF2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human GRF2 aa 50-250.
Database link: Q13905 -
Positive control
- IHC-P: Human skeletal muscle and placenta tissue. WB: GRF2 overexpression HEK-293T cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 121 kDa.
|
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 121 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. -
Tissue specificity
Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver. -
Sequence similarities
Contains 1 N-terminal Ras-GEF domain.
Contains 1 Ras-GEF domain. - Information by UniProt
-
Database links
- Entrez Gene: 2889 Human
- Omim: 600303 Human
- SwissProt: Q13905 Human
- Unigene: 127897 Human
-
Form
It localizes to cytoplasm and endosomes. -
Alternative names
- C3G antibody
- C3G protein antibody
- CRK SH3 binding GNRP antibody
see all
Images
-
Formalin-fixed, paraffin-embedded human placenta tissue stained for RAPGEF1 with ab251683 at a 1/50 dilution in immunohistochemical analysis.
-
All lanes : Anti-GRF2 antibody (ab251683) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : GRF2 overexpression HEK-293T cell lysate
Predicted band size: 121 kDa -
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for RAPGEF1 with ab251683 at a 1/50 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for RAPGEF1 with ab251683 at a 1/50 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human liver tissue shows absence of immunoreactivity for RAPGEF1 when incubated with ab251683 at a 1/50 dilution (negative control).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab251683 has not yet been referenced specifically in any publications.