Overview

  • Product name

    Anti-GRIM19 antibody [6E1BH7]
    See all GRIM19 primary antibodies
  • Description

    Mouse monoclonal [6E1BH7] to GRIM19
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, ELISA, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • Human heart, Bovine heart, Rat heart, and Mouse heart isolated mitochondria, Human fibroblasts, HeLa cells
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

     

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use a concentration of 8 µg/ml. In-Cell ELISA (0.8 µg/well)
Flow Cyt Use a concentration of 1 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes.
  • Tissue specificity

    Widely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis.
  • Involvement in disease

    Defects in NDUFA13 may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
  • Sequence similarities

    Belongs to the complex I NDUFA13 subunit family.
  • Developmental stage

    Expressed in numerous fetal tissues.
  • Cellular localization

    Mitochondrion inner membrane. Nucleus. May be translocated into the nucleus upon IFN/RA treatment.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2700054G14Rik antibody
    • AU022060 antibody
    • B16.6 antibody
    • CDA016 antibody
    • Cell death regulatory protein antibody
    • Cell death regulatory protein GRIM-19 antibody
    • CGI-39 antibody
    • CGI39 protein antibody
    • CI-B16.6 antibody
    • Complex I-B16.6 antibody
    • FLJ58045 antibody
    • FLJ59191 antibody
    • Gene associated with retinoic and IFN-induced mortality 19 protein antibody
    • Gene associated with retinoic and interferon-induced mortality 19 protein antibody
    • Gene associated with retinoic interferon induced mortality 19 protein antibody
    • GRIM-19 antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 antibody
    • NADH ubiquinone oxidoreductase B16.6 subunit antibody
    • NADH-ubiquinone oxidoreductase B16.6 subunit antibody
    • NDUAD_HUMAN antibody
    • NDUFA13 antibody
    • RGD1565358 antibody
    see all

Images

  • All lanes : Anti-GRIM19 antibody [6E1BH7] (ab110240) at 1 µg/ml

    Lane 1 : Human heart mitochondria
    Lane 2 : Bovine heart mitochondria
    Lane 3 : Rat heart mitochondria
    Lane 4 : Mouse heart mitochondria

    Predicted band size: 17 kDa

  • Passively cleared 250 μm thick human cerebellar sections were stained for neurofilament H (blue), GRIM19 (NDUFA13) (ab110240, 1/100) (red), COX4 (ab110261, 1/100) (green) and Myelin basic protein (purple). Mitochondria are clearly visible through the positive labelling of GRIM19 (NDUFA13) (ab110240, 1/100) (red) and COX4 (ab110261, 1/100) (green). The myelin sheaths (Myelin basic protein, 647) of the Purkinje cell axons have been clearly labelled. In the 405 nm channel there is a high level of autofluorescence making it difficult to visualise Purkinje cell axons (neurofilament H). The presence of autofluorescence in the 405 nm and 488 nm channel was confirmed through a no primary antibody control section. Scale: 100 μm.

  • 250 μm-thick formalin-fixed cerebellum section were passively cleared using PACT and immunofluorescently labelled to identify mitochondrial mass (porin (ab14734, 1/100) and SDHA (ab14715, 1/100), 647 nm) and complex I subunits within the mitochondrial respiratory chain (NDUFB8 (ab110242, 1/100) and NDUFA13 (ab110240, 1/100); 546 nm) in conjunction with a neuronal marker (NF-H; 488 nm) in control 1. Scale: 100 μm.

  • Mitochondrial localization of GRIM19 visualized by immunocytochemistry using ab110240 at a concentration of 1 µg/mL. Cultured Human fibroblasts were fixed, permeabilized and then labeled with ab110240 followed by Alexa® 488 goat-anti-mouse IgG.
  • HeLa cells were stained with 1 µg/mL ab110240 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • ab110240 staining GRIM19 in Human cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/3000 in TBS) for 1 hour and stained with DAB.

    See Abreview

References

This product has been referenced in:

  • Rouzier C  et al. NDUFS6 related Leigh syndrome: a case report and review of the literature. J Hum Genet N/A:N/A (2019). Read more (PubMed: 30948790) »
  • Pulman J  et al. Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement. Hum Mol Genet 28:1445-1462 (2019). Read more (PubMed: 30566640) »
See all 29 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HEK293
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Neutral buffered formalin

Abcam user community

Verified customer

Submitted May 17 2019

Answer

Thank you for contacting us.

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I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain - Cerebellum)
Specification
Brain - Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No

Ms. Philippa Hepplewhite

Verified customer

Submitted Jun 23 2012

Application
Western blot
Sample
Mouse Cell lysate - other (Isolated Mouse Liver Mitochondria)
Loading amount
20 µg
Specification
Isolated Mouse Liver Mitochondria
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 05 2011

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