Anti-GRIP1 antibody (ab25963)
Key features and details
- Rabbit polyclonal to GRIP1
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-GRIP1 antibody
See all GRIP1 primary antibodies -
Description
Rabbit polyclonal to GRIP1 -
Host species
Rabbit -
Specificity
This antibody is raised against glutamate receptor-interacting protein 1 -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human -
Immunogen
Synthetic peptide corresponding to Mouse GRIP1 aa 1050 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 135 kDa (predicted molecular weight: 122 kDa).
Abcam recommends using BSA as the blocking agent. |
Notes |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 135 kDa (predicted molecular weight: 122 kDa). Abcam recommends using BSA as the blocking agent. |
Target
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Function
May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons. -
Sequence similarities
Contains 7 PDZ (DHR) domains. -
Domain
PDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1 (By similarity). PDZ 4 and PDZ 5 mediate interaction with PRLHR. -
Cellular localization
Cytoplasmic vesicle. Endoplasmic reticulum. Cell junction > synapse > postsynaptic cell membrane. Cytoplasmic and membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities. - Information by UniProt
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Database links
- Entrez Gene: 23426 Human
- Entrez Gene: 74053 Mouse
- Entrez Gene: 84016 Rat
- Omim: 604597 Human
- SwissProt: Q9Y3R0 Human
- SwissProt: Q925T6 Mouse
- SwissProt: P97879 Rat
- Unigene: 505946 Human
see all -
Alternative names
- as GRIP antibody
- Glutamate receptor-interacting protein 1 antibody
- GRIP-1 antibody
see all
Images
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All lanes : Anti-GRIP1 antibody (ab25963) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate - postnatal day 7
Lane 2 : Brain (Rat) Tissue Lysate - normal adult tissue
Lane 3 : Brain (Rat) Tissue Lysate - postnatal day 7
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 122 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?
Additional bands at: 108 kDa, 38 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25963 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab25963 has been referenced in 2 publications.
- Barnes SJ et al. Deprivation-Induced Homeostatic Spine Scaling In Vivo Is Localized to Dendritic Branches that Have Undergone Recent Spine Loss. Neuron 96:871-882.e5 (2017). IHC-P . PubMed: 29107520
- Gainey MA et al. Activity-dependent synaptic GRIP1 accumulation drives synaptic scaling up in response to action potential blockade. Proc Natl Acad Sci U S A 112:E3590-9 (2015). PubMed: 26109571