Recombinant Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4902] to CXCL1/GRO alpha + GRO gamma + GRO beta
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902]
See all CXCL1/GRO alpha+GRO gamma+GRO beta primary antibodies -
Description
Rabbit monoclonal [EPR4902] to CXCL1/GRO alpha + GRO gamma + GRO beta -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human GRO alpha aa 50-150. The exact sequence is proprietary.
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Positive control
- Human GRO alpha recombinant protein.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4902 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 8 kDa (predicted molecular weight: 11 kDa).
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 8 kDa (predicted molecular weight: 11 kDa). |
Target
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Cellular localization
CXCL1/GRO alpha: Secreted. GRO gamma: Secreted. -
Database links
- Entrez Gene: 2919 Human
- Entrez Gene: 2921 Human
- Omim: 139111 Human
- Omim: 155730 Human
- SwissProt: P09341 Human
- SwissProt: P19875 Human
- SwissProt: P19876 Human
- Unigene: 708652 Human
see all
Images
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All lanes : Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898) at 1/2000 dilution
Lane 1 : Human GRO alpha active protein
Lane 2 : Human GRO beta fragment recombinant protein
Lane 3 : Human GRO gamma fragment recombinant protein
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 10,36,10 kDa why is the actual band size different from the predicted?Exposure time: 3 minutes
Blocking buffer and concentartion:5% NFDM/TBST
Diluting buffer and concentration:5% NFDM/TBST
Human GRO beta fragment protein contain aa35-107 with a GST-Tage.Human GRO gamma fragment recombinant protein contain aa35-107 with a His-Tag.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab124898 has been referenced in 1 publication.
- Pasquier J et al. Circulating microparticles in acute diabetic Charcot foot exhibit a high content of inflammatory cytokines, and support monocyte-to-osteoclast cell induction. Sci Rep 7:16450 (2017). PubMed: 29180664