Anti-GSAP antibody (ab106630)
Key features and details
- Rabbit polyclonal to GSAP
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-GSAP antibody -
Description
Rabbit polyclonal to GSAP -
Host species
Rabbit -
Specificity
ab106630 is predicted to not cross-react with other F-box protein family members. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Human GSAP (C terminal).
-
Positive control
- WB: EL4 cell lysate. IHC-P: Mouse brain tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab106630 was purified by affinity chromatography via a peptide column. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
WB |
Use a concentration of 0.25 µg/ml. Predicted molecular weight: 98 kDa.
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 0.25 µg/ml. Predicted molecular weight: 98 kDa. |
Target
-
Function
Regulator of gamma-secretase activity, which specifically activates the production of beta-amyloid protein (beta-amyloid protein 40 and beta-amyloid protein 42), without affecting the cleavage of other gamma-secretase targets such has Notch. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Specifically promotes the gamma-cleavage of APP CTF-alpha (also named APP-CTF) by the gamma-secretase complex to generate beta-amyloid, while it reduces the epsilon-cleavage of APP CTF-alpha, leading to a low production of AICD. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the GSAP family. -
Post-translational
modificationsThe protein is first synthesized as a holoprotein form of 98 kDa and rapidly processed into the gamma-secretase-activating protein 16 kDa C-terminal form, which constitutes the predominant form. -
Cellular localization
Golgi apparatus > trans-Golgi network. - Information by UniProt
-
Database links
- Entrez Gene: 212167 Mouse
- SwissProt: Q3TCV3 Mouse
- Unigene: 121705 Mouse
-
Alternative names
- A530088I07Rik antibody
- DKFZp667B242 antibody
- Gamma-secretase-activating protein 16 kDa C-terminal form antibody
see all
Images
-
All lanes : Anti-GSAP antibody (ab106630) at 0.25 µg/ml
Lane 1 : EL4 cell lysate
Lane 2 : EL4 cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 98 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse brain tissue staining GSAP with ab106630 at 20µg/ml
-
Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling GSAP with Anti-GSAP antibody (ab106630) at 5μg/ml.
Protocols
Datasheets and documents
-
Datasheet download
References (3)
ab106630 has been referenced in 3 publications.
- Jin C et al. Modulation of amyloid precursor protein cleavage by γ-secretase activating protein through phase separation. Proc Natl Acad Sci U S A 119:e2122292119 (2022). PubMed: 35298330
- Hajjari SN et al. MicroRNA-4422-5p as a Negative Regulator of Amyloidogenic Secretases: A Potential Biomarker for Alzheimer's Disease. Neuroscience 463:108-115 (2021). PubMed: 33836245
- Wang L et al. PTH-induced EndMT via miR-29a-5p/GSAP/Notch1 pathway contributed to valvular calcification in rats with CKD. Cell Prolif 54:e13018 (2021). PubMed: 33945189