Biotin Anti-Ghrelin antibody (ab48285)
Key features and details
- Biotin Rabbit polyclonal to Ghrelin
- Suitable for: ELISA, IP, IHC-P, RIA
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Ghrelin antibody
See all Ghrelin primary antibodies -
Description
Biotin Rabbit polyclonal to Ghrelin -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: ELISA, IP, IHC-P, RIAmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cat, Dog, Pig, Baboon, Rhesus monkey, Gerbil -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.73% PBS, 50% Glycerol (glycerin, glycerine), 0.25% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab48285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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RIA |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
RIA
Use at an assay dependent concentration. |
Target
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Function
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility. -
Tissue specificity
Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy. -
Sequence similarities
Belongs to the motilin family. -
Post-translational
modificationsO-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
Amidation of Leu-98 is essential for obestatin activity. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 51738 Human
- Entrez Gene: 58991 Mouse
- Entrez Gene: 59301 Rat
- Omim: 605353 Human
- SwissProt: Q9UBU3 Human
- SwissProt: Q9EQX0 Mouse
- SwissProt: Q9GKY5 Pig
- SwissProt: Q9QYH7 Rat
see all -
Alternative names
- Appetite regulating hormone antibody
- Ghrelin 27 antibody
- Ghrelin 28 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab48285 has been referenced in 1 publication.
- Stojsavljevic-Shapeski S et al. Expression of adipokine ghrelin and ghrelin receptor in human colorectal adenoma and correlation with the grade of dysplasia. World J Gastrointest Surg 13:1708-1720 (2021). PubMed: 35070075