Anti-GIV antibody (ab113890)
Key features and details
- Rabbit polyclonal to GIV
- Suitable for: ELISA, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GIV antibody
See all GIV primary antibodies -
Description
Rabbit polyclonal to GIV -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human GIV conjugated to keyhole limpet haemocyanin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 216 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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Notes |
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ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 216 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation (By similarity). Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome. -
Tissue specificity
Expressed ubiquitously. -
Sequence similarities
Belongs to the CCDC88 family. -
Post-translational
modificationsPhosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for the delocalization from the cell membrane and for cell migration. -
Cellular localization
Membrane. Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Cell projection > lamellipodium. Localizes to the cell membrane through interaction with phosphoinositides. - Information by UniProt
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Database links
- Entrez Gene: 55704 Human
- Entrez Gene: 108686 Mouse
- Entrez Gene: 305605 Rat
- Omim: 609736 Human
- SwissProt: Q3V6T2 Human
- SwissProt: Q5SNZ0 Mouse
- Unigene: 292925 Human
- Unigene: 338284 Mouse
see all -
Alternative names
- AKT iphosphorylation enhancer antibody
- Akt phosphorylation enhancer antibody
- APE antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab113890 has been referenced in 3 publications.
- Dang J et al. Roles of metformin-mediated girdin expression in metastasis of epithelial ovarian cancer. Cell Biol Int 45:1030-1037 (2021). PubMed: 33404163
- Lan Y et al. Long noncoding RNA MEG3 prevents vascular endothelial cell senescence by impairing miR-128-dependent Girdin downregulation. Am J Physiol Cell Physiol 316:C830-C843 (2019). PubMed: 30576236
- Yang Z et al. Girdin protein: A potential metastasis predictor associated with prognosis in lung cancer. Exp Ther Med 15:2837-2843 (2018). PubMed: 29456687