Anti-Gliadin antibody [14D5] (ab36729)
Key features and details
- Mouse monoclonal [14D5] to Gliadin
- Suitable for: WB
- Reacts with: Wheat, Hordeum vulgare
- Isotype: IgG2a
Overview
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Product name
Anti-Gliadin antibody [14D5] -
Description
Mouse monoclonal [14D5] to Gliadin -
Host species
Mouse -
Specificity
This antibody reacts specifically with the deamidated peptide (KLQPFPQPELPYPQPQ) when coated directly in an immunoplate. Does not react with Oats. This antibody reacts strongly with the deamidated peptide but cross-reacts about 5% with the non-deamidated peptide. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Wheat, Hordeum vulgare, Secale cereale -
Immunogen
Gliadin-related peptide Lys57-Glu65-[alpha-gliadin (58-73)]
KLQPFPQPELPYPQPQ
) coupled to immunogenic carrier protein. -
Epitope
The epitope is located in the Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ) peptide. -
General notes
The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin. -
Clonality
Monoclonal -
Clone number
14D5 -
Myeloma
x63-Ag8.653 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000.
dilution 1/1000 |
Notes |
---|
WB
1/1000. dilution 1/1000 |
Target
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Relevance
Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The alcohol soluble proteins (prolamins) from wheat, rye, barley and oats produce the harmful effect of coeliac disease or gluten sensitive enteropathy in humans by causing characteristic changes in the intestinal mucosa. Patients so affected must avoid eating these grains and replace them with rice, corn, potatoes, etc. Many gluten-free foods are produced industrially, thus several immunoassays have been developed for determination of gliadin in supposedly gluten-free foods. -
Cellular localization
Cytoplasmic; major seed storage protein in wheat. -
Database links
- Entrez Gene: 543191 Wheat
- SwissProt: P02863 Wheat
- SwissProt: P04721 Wheat
- SwissProt: P04722 Wheat
- SwissProt: P04723 Wheat
- SwissProt: P04724 Wheat
- SwissProt: P04725 Wheat
- SwissProt: P04726 Wheat
see all -
Alternative names
- prolamin antibody
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab36729 has been referenced in 3 publications.
- Méndez PX et al. The Ability of the Yeast Wickerhamomyces anomalus to Hydrolyze Immunogenic Wheat Gliadin Proteins. Foods 11:N/A (2022). PubMed: 36553848
- Manai F et al. The In Vitro Effects of Enzymatic Digested Gliadin on the Functionality of the Autophagy Process. Int J Mol Sci 19:N/A (2018). ICC/IF . PubMed: 29473905
- Engstrom N et al. Towards Celiac-safe foods: Decreasing the affinity of transglutaminase 2 for gliadin by addition of ascorbyl palmitate and ZnCl2 as detoxifiers. Sci Rep 7:77 (2017). PubMed: 28250436