Name: Anti-Glucagon antibody [EP3070]
Brand: RabMAb
SKU: ab92517
Rating: 5 (4 reviews)

Product name

Anti-Glucagon antibody [EP3070]
See all Glucagon primary antibodies
Description

Rabbit monoclonal [EP3070] to Glucagon
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, IHC-Fr, ELISAmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human Glucagon aa 50-150 (N terminal). The exact sequence is proprietary.
Positive control
- IHC-P: Murine, human, and rat pancreatic tissue. WB: Capan-1 cell lysate, Mouse and rat pancreas tissue lysate
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP3070
Isotype

IgG
Research areas

Alternative Versions
- Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Recombinant Pig Oxyntomodulin protein (ab73342)

Our Abpromise guarantee covers the use of ab92517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P		1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/1000 - 1/5000
IHC-Fr		Use at an assay dependent concentration. Abreview 62874
ELISA		Use at an assay dependent concentration.

Relevance

Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth.
Cellular localization

Secreted
Database links
- Entrez Gene: 2641 Human
- Entrez Gene: 14526 Mouse
- Entrez Gene: 24952 Rat
- Omim: 138030 Human
- SwissProt: P01275 Human
- SwissProt: P55095 Mouse
- SwissProt: P06883 Rat
- Unigene: 516494 Human
- Unigene: 45494 Mouse
- Unigene: 54383 Rat
see all
Alternative names
- GCG antibody

Immunohistochemistry (Frozen sections) - Anti-Glucagon antibody [EP3070] (ab92517)This image is courtesy of an anonymous Abreview.

Frozen mouse pancreas tissue stained for Glucagon (green) using ab92517 at 1/100 dilution in immunohistochemical analysis, followed by Donkey anti-rabbit IgG Alexa Fluor 488.

Insulin (red), counterstained with DAPI (blue).
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)Image from Oelze M et al., PLoS One. 2014;9(11):e112394. Fig 1.; doi: 10.1371/journal.pone.0112394.

Glucagon measurements in controls and diabetic rats 8 weeks after STZ injection and 7 weeks of SGLT2i (low and high dose) treatment. Representative immunohistochemical stainings of pancreatic tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat pancreas tissue sections labeling Glucagon with Purified ab92517 at 1:8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0)Goat Anti-Rabbit IgG H&L (HRP) ab97051was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Western blot - Anti-Glucagon antibody [EP3070] (ab92517)

Anti-Glucagon antibody [EP3070] (ab92517) at 0.3 µg/ml (purified) + Capan-1 (Human pancreas adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 21 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse pancreas tissue sections labeling Glucagon with Purified ab92517 at 1:8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0)Goat Anti-Rabbit IgG H&L (HRP) ab97051was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Western blot - Anti-Glucagon antibody [EP3070] (ab92517)

Anti-Glucagon antibody [EP3070] (ab92517) at 0.3 µg/ml (purified) + Mouse pancreas lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 21 kDa
Observed band size: 21 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas tissue sections labeling Glucagon with Purified ab92517 at 1:8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0)Goat Anti-Rabbit IgG H&L (HRP) ab97051was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Western blot - Anti-Glucagon antibody [EP3070] (ab92517)

Anti-Glucagon antibody [EP3070] (ab92517) at 0.3 µg/ml (purified) + Rat pancreas lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 21 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)Image is courtesy of an anonymous AbReview.

Immunohistochemical analysis of PFA-fixed paraffin-embedded murine pancreatic tissue sections, labelling glucagon with unpurified ab92517 at a dilution of 1/2500 incubated for 18 hours at 4°C. Antigen retrival was performed through heat-mediated 10mM sodium citrate pH 6.0 incubation. Blocking was done with 5% serum for 1 hour at 25°C. The secondary was a donkey anti-rabbit polyclonal Alexa Fluor^® 488 conjugate at 1/400. Counterstaining is with DAPI (blue) against nuclear DNA.
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)

Unpurified ab92517, at 1/1000 dilution, staining Glucagon in paraffin embedded Human pancreas tissue by Immunohistochemistry.

Western blot protocols
Immunohistochemistry protocols

This product has been referenced in:

Monazzam A et al. Increased Expression of GLP-1R in Proliferating Islets of Men1 Mice is Detectable by [68Ga]Ga-DO3A-VS-Cys40-Exendin-4 /PET. Sci Rep 8:748 (2018). IHC ; Mouse . Read more (PubMed: 29335487) »
Prabakaran AD et al. Identification of early indicators of altered metabolism in normal development using a rodent model system. Dis Model Mech 11:N/A (2018). Read more (PubMed: 29434026) »

See all 18 Publications for this product

Publishing research using ab92517? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (pancreas)

Permeabilization

Specification

pancreas

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 22 2017

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Pancreas)

Permeabilization

Specification

Pancreas

Blocking step

serum +1% BSA in 0.1% PBST as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 24 2017

Application

ELISA

Sample

Human Recombinant protein (Synthetic peptide)

Specification

Synthetic peptide

Blocking step

BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Type

Indirect

Abcam user community

Verified customer

Submitted Mar 01 2016

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (pancreas)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10mm sodium citric PH 6.0

Permeabilization

Specification

pancreas

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 12 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-Glucagon antibody [EP3070] (ab92517)

Overview

Properties

Associated products

Applications

Target

Images

Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

Immunohistochemistry (Frozen sections) abreview for Anti-Glucagon antibody [EP3070]

Immunohistochemistry (Frozen sections) abreview for Anti-Glucagon antibody [EP3070]

ELISA abreview for Anti-Glucagon antibody [EP3070]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucagon antibody [EP3070]