Name: Anti-Glucagon antibody [EP3070]
Brand: RabMAb
SKU: ab92517
Rating: 5 (5 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP3070] to Glucagon
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Glucagon antibody [EP3070]
See all Glucagon primary antibodies
Description

Rabbit monoclonal [EP3070] to Glucagon
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human Glucagon aa 50-150 (N terminal). The exact sequence is proprietary.
Positive control
- IHC-P: Murine, human, and rat pancreatic tissue. IHC-Fr: Mouse pancreas tissue. WB: Capan-1 cell lysate, Mouse and rat pancreas tissue lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP3070
Isotype

IgG
Research areas

Alternative Versions
- Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human Glucagon protein (ab109948)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(1)	1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P	(1)	1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/1000 - 1/5000.

Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P 1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/1000 - 1/5000.

Relevance

Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth.
Cellular localization

Secreted
Database links
- Entrez Gene: 2641 Human
- Entrez Gene: 14526 Mouse
- Entrez Gene: 24952 Rat
- Omim: 138030 Human
- SwissProt: P01275 Human
- SwissProt: P55095 Mouse
- SwissProt: P06883 Rat
- Unigene: 516494 Human
- Unigene: 45494 Mouse
- Unigene: 54383 Rat
see all
Alternative names
- GCG antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)Image from Oelze M et al., PLoS One. 2014;9(11):e112394. Fig 1.; doi: 10.1371/journal.pone.0112394. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Glucagon measurements in controls and diabetic rats 8 weeks after STZ injection and 7 weeks of SGLT2i (low and high dose) treatment. Representative immunohistochemical stainings of pancreatic tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat pancreas tissue sections labeling Glucagon with Purified ab92517 at 1:8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0)Goat Anti-Rabbit IgG H&L (HRP) ab97051was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Western blot - Anti-Glucagon antibody [EP3070] (ab92517)

Anti-Glucagon antibody [EP3070] (ab92517) at 0.3 µg/ml (purified) + Capan-1 (Human pancreas adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 21 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse pancreas tissue sections labeling Glucagon with Purified ab92517 at 1:8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0)Goat Anti-Rabbit IgG H&L (HRP) ab97051was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Western blot - Anti-Glucagon antibody [EP3070] (ab92517)

Anti-Glucagon antibody [EP3070] (ab92517) at 0.3 µg/ml (purified) + Mouse pancreas lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 21 kDa
Observed band size: 21 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas tissue sections labeling Glucagon with Purified ab92517 at 1:8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0)Goat Anti-Rabbit IgG H&L (HRP) ab97051was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Western blot - Anti-Glucagon antibody [EP3070] (ab92517)

Anti-Glucagon antibody [EP3070] (ab92517) at 0.3 µg/ml (purified) + Rat pancreas lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 21 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)Image is courtesy of an anonymous AbReview.

Immunohistochemical analysis of PFA-fixed paraffin-embedded murine pancreatic tissue sections, labelling glucagon with unpurified ab92517 at a dilution of 1/2500 incubated for 18 hours at 4°C. Antigen retrival was performed through heat-mediated 10mM sodium citrate pH 6.0 incubation. Blocking was done with 5% serum for 1 hour at 25°C. The secondary was a donkey anti-rabbit polyclonal Alexa Fluor^® 488 conjugate at 1/400. Counterstaining is with DAPI (blue) against nuclear DNA.
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)

Unpurified ab92517, at 1/1000 dilution, staining Glucagon in paraffin embedded Human pancreas tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-Glucagon antibody [EP3070] (ab92517)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab92517? Please let us know so that we can cite the reference in this datasheet.

ab92517 has been referenced in 26 publications.

Sayers SR et al. Structure-functional changes in eNAMPT at high concentrations mediate mouse and human beta cell dysfunction in type 2 diabetes. Diabetologia 63:313-323 (2020). PubMed: 31732790
Campbell SA et al. Human islets contain a subpopulation of glucagon-like peptide-1 secreting a cells that is increased in type 2 diabetes. Mol Metab 39:101014 (2020). PubMed: 32413586
Ramadori G et al. S100A9 extends lifespan in insulin deficiency. Nat Commun 10:3545 (2019). PubMed: 31391467
Holm LJ et al. Fenofibrate increases very-long-chain sphingolipids and improves blood glucose homeostasis in NOD mice. Diabetologia N/A:N/A (2019). PubMed: 31410530
Kim KS et al. Glycemic effect of pancreatic preproglucagon in mouse sleeve gastrectomy. JCI Insight 4:N/A (2019). PubMed: 31619587

View all Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Application

Western blot

Sample

Human Recombinant protein (CHO-S)

Gel Running Conditions

Reduced Denaturing (8% gel)

Loading amount

0.5 µg

Specification

CHO-S

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Mrs. Min Sun Kim

Verified customer

Submitted Jul 01 2020

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (pancreas)

Permeabilization

Specification

pancreas

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 22 2017

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Pancreas)

Permeabilization

Specification

Pancreas

Blocking step

serum +1% BSA in 0.1% PBST as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 24 2017

Application

ELISA

Sample

Human Recombinant protein (Synthetic peptide)

Specification

Synthetic peptide

Blocking step

BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Type

Indirect

Abcam user community

Verified customer

Submitted Mar 01 2016

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (pancreas)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10mm sodium citric PH 6.0

Permeabilization

Specification

pancreas

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 12 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Relevance

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (26)

Customer reviews and Q&As

Western blot abreview for Anti-Glucagon antibody [EP3070]

Immunohistochemistry (Frozen sections) abreview for Anti-Glucagon antibody [EP3070]

Immunohistochemistry (Frozen sections) abreview for Anti-Glucagon antibody [EP3070]

ELISA abreview for Anti-Glucagon antibody [EP3070]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucagon antibody [EP3070]