Anti-Glucocorticoid Receptor beta antibody (ab3581)
Key features and details
- Rabbit polyclonal to Glucocorticoid Receptor beta
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-Glucocorticoid Receptor beta antibody
See all Glucocorticoid Receptor beta primary antibodies -
Description
Rabbit polyclonal to Glucocorticoid Receptor beta -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Glucocorticoid Receptor beta aa 728-742.
Sequence:NVMWLKPESTSHTLI
(Peptide available asab39765) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).Can be blocked with Human Glucocorticoid Receptor beta peptide (ab39765). Sample preparation: Lysates boiled in reducing buffer for 5 min. 20µg of protein loaded
Blocking agent: 5% non-fat milk in Tris buffered saline (TBS)
Primary antibody: overnight at 4°C
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Notes |
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WB
1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).Can be blocked with Human Glucocorticoid Receptor beta peptide (ab39765). Sample preparation: Lysates boiled in reducing buffer for 5 min. 20µg of protein loaded Blocking agent: 5% non-fat milk in Tris buffered saline (TBS) Primary antibody: overnight at 4°C |
Target
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Function
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation. -
Tissue specificity
Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. -
Involvement in disease
Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsIncreased proteasome-mediated degradation in response to glucocorticoids.
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
Sumoylated; this reduces transcription transactivation.
Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2908 Human
- SwissProt: P04150 Human
- Unigene: 122926 Human
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Alternative names
- GCCR antibody
- GCR antibody
- GCR_HUMAN antibody
see all
Images
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Anti-Glucocorticoid Receptor beta antibody (ab3581) + hGR beta transfected COS-7 whole cell extract
Predicted band size: 83 kDa -
All lanes : Anti-Glucocorticoid Receptor beta antibody (ab3581) at 1/500 dilution
Lane 1 : COS-74 whole cell extract transfected with Glucocorticoid Receptor alpha
Lane 2 : COS-74 whole cell extract transfected with Glucocorticoid Receptor beta
Predicted band size: 83 kDa
Protocols
Datasheets and documents
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Datasheet download
References (15)
ab3581 has been referenced in 15 publications.
- Lo CY et al. Increased Interleukin-17 and Glucocorticoid Receptor-β Expression in Interstitial Lung Diseases and Corticosteroid Insensitivity. Front Immunol 13:905727 (2022). PubMed: 35865549
- Saif Z et al. A preferential switch between placental GR exon 1 promoter variants in the presence of maternal asthma or inflammation upregulates GRa D isoforms. Placenta 108:64-72 (2021). PubMed: 33819863
- Kolm I et al. Lichen aureus with pseudolymphomatous infiltrate. J Cutan Pathol 48:669-673 (2021). PubMed: 33368548
- Bernays V et al. Glucocorticoid Receptor Beta and Its Prognostic Value on Treatment Response in Chronic Vulvar Dermatitis. Skin Pharmacol Physiol 34:30-37 (2021). PubMed: 33592607
- Xu W et al. Huai Qi Huang Potentiates Dexamethasone-Mediated Lethality in Acute Lymphoblastic Leukemia Cells by Upregulating Glucocorticoid Receptor a. Med Sci Monit 26:e921649 (2020). PubMed: 32065117