Glucose Assay Kit - reducing agent compatible (ab102517)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.02 mM
Overview
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Product name
Glucose Assay Kit - reducing agent compatible
See all Glucose kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 0.02 mM -
Range
0.02 mM - 10 mM -
Assay time
0h 30m -
Product overview
Glucose Assay Kit ab102517 provides direct measurement of glucose in biological samples. It is particularly suitable for serum and urine samples since it is unaffected by reducing substances, which can interfere with detection in oxidase-based kits.
In the glucose assay protocol, glucose is specifically oxidized to generate a product which reacts with a dye to generate color (λ = 450 nm) whose intensity is proportional to glucose concentration.
The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes.
The kit can detect glucose concentrations in the range of 20µM-10mM.
Glucose assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with a microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K686 Glucose Colorimetric Assay Kit II. K686-100 is the same size as the 100 test size of ab102517.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Glucose Assay Buffer WM 1 x 25ml Glucose Enzyme Mix Green 1 unit Glucose Standard Yellow 1 x 100µl Glucose Substrate Mix Red 1 unit -
Research areas
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Relevance
Glucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.
Images
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Functional Studies - Glucose Detection Kit (ab102517)
Glucose measured in biological fluids. Human samples diluted 20-80 fold. Mouse samples diluted 1-27 fold.
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Functional Studies - Glucose Detection Kit (ab102517)Standard curve: mean of duplicates (+/- SD) with background reads subtracted -
Functional Studies - Glucose Detection Kit (ab102517)Standard curve for glucose run using the kit protocol
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab102517 has been referenced in 8 publications.
- Christofi M et al. Low-dose 2-deoxy glucose stabilises tolerogenic dendritic cells and generates potent in vivo immunosuppressive effects. Cell Mol Life Sci 78:2857-2876 (2021). PubMed: 33074350
- Woo M et al. Effects of collagen peptides from skate (Raja kenojei) skin on improvements of the insulin signaling pathway via attenuation of oxidative stress and inflammation. Food Funct 11:2017-2025 (2020). PubMed: 32096813
- Grassi ES et al. Hypoxia-induced release, nuclear translocation, and signaling activity of a DLK1 intracellular fragment in glioma. Oncogene 39:4028-4044 (2020). PubMed: 32205867
- Cabral DJ et al. Microbial Metabolism Modulates Antibiotic Susceptibility within the Murine Gut Microbiome. Cell Metab 30:800-823.e7 (2019). PubMed: 31523007
- Collin de l'Hortet A et al. Generation of Human Fatty Livers Using Custom-Engineered Induced Pluripotent Stem Cells with Modifiable SIRT1 Metabolism. Cell Metab 30:385-401.e9 (2019). PubMed: 31390551
- Heimlicher MB et al. Reversible solidification of fission yeast cytoplasm after prolonged nutrient starvation. J Cell Sci 132:N/A (2019). PubMed: 31558680
- Feaver RE et al. Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis. JCI Insight 1:e90954 (2016). PubMed: 27942596
- Jou YC et al. Foxp3 enhances HIF-1a target gene expression in human bladder cancer through decreasing its ubiquitin-proteasomal degradation. Oncotarget 7:65403-65417 (2016). PubMed: 27557492