Recombinant Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10508(N)] to Glucose Transporter GLUT3 + GLUT14 - N-terminal
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal
See all Glucose Transporter GLUT3+GLUT14 primary antibodies -
Description
Rabbit monoclonal [EPR10508(N)] to Glucose Transporter GLUT3 + GLUT14 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain and Rat brain tissue lysates; SHSY-5Y, U87-MG, NIH3T3, HepG2, C6 and RAW264.7 cell lysates, GST-tagged Recombinant Human SLC2A3(GLUT3) protein (Full length), GST tagged Recombinant Human SLC2A14(GLUT14) protein (aa51-105)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10508(N) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
Target
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Cellular localization
Glucose Transporter GLUT3: Membrane. GLUT14: Membrane. -
Database links
- Entrez Gene: 144195 Human
- Entrez Gene: 6515 Human
- Entrez Gene: 20527 Mouse
- Entrez Gene: 25551 Rat
- Omim: 138170 Human
- Omim: 611039 Human
- SwissProt: P11169 Human
- SwissProt: Q8TDB8 Human
see all
Images
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All lanes : Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071) at 1/1000 dilution
Lane 1 : GST-tagged Recombinant Human SLC2A3(GLUT3) protein (Full length)
Lane 2 : GST tagged Recombinant Human SLC2A14(GLUT14) protein (aa51-105)
Lane 3 : MYC and DDK-tagged Recombinant Human SLC2A1(GLUT1) protein (Full length)
Lane 4 : MYC and DDK-tagged Recombinant Human SLC2A4(GLUT4) protein (Full length)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 54 kDa
Observed band size: 38,70 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 15 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
We are unsure as to the identity of the band below 54kDa. This is not the target band.
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All lanes : Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071) at 1/10000 dilution
Lane 1 : SH-SHY5Y cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa -
All lanes : Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : RAW264.7 cell lysate
Lane 3 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (23)
ab191071 has been referenced in 23 publications.
- Laferrière F et al. Similar neuronal imprint and no cross-seeded fibrils in α-synuclein aggregates from MSA and Parkinson's disease. NPJ Parkinsons Dis 8:10 (2022). PubMed: 35027576
- Li X et al. Ajuba Overexpression Promotes Breast Cancer Chemoresistance and Glucose Uptake through TAZ-GLUT3/Survivin Pathway. Biomed Res Int 2022:3321409 (2022). PubMed: 35178446
- Zhang R et al. An NF-κB/OVOL2 circuit regulates glucose import and cell survival in non-small cell lung cancer. Cell Commun Signal 20:40 (2022). PubMed: 35346238
- Jacquier V et al. RIP140 inhibits glycolysis-dependent proliferation of breast cancer cells by regulating GLUT3 expression through transcriptional crosstalk between hypoxia induced factor and p53. Cell Mol Life Sci 79:270 (2022). PubMed: 35501580
- Philips T et al. MCT1 Deletion in Oligodendrocyte Lineage Cells Causes Late-Onset Hypomyelination and Axonal Degeneration. Cell Rep 34:108610 (2021). PubMed: 33440165