Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Urine
Product nameGlutamate Assay Kit
See all Glutamate kits
Sample typeUrine, Serum, Plasma, Other biological fluids, Cell Lysate
Assay time0h 40m
Glutamate Assay Kit ab83389 provides a sensitive detection method of the glutamate in a variety of samples.
The glutamate assay kit will only measure free glutamate levels but not glutamic acid found in the backbone of peptides or proteins.
In the glutamate assay protocol, the enzyme mix recognizes glutamate as a specific substrate leading to proportional color development.The amount of glutamate can therefore be easily quantified by colorimetric spectrophotometry at OD = 450 nm.
Glutamate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at 37ºC
- analyze with microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K629 Glutamate Colorimetric Assay Kit. K629-100 is the same size as the 100 test size of ab83389.
EDTA plasma may not be used with the ab83389 Glutamate Assay Kit. EDTA can interfere with the assay.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Glutamate Assay Buffer WM 1 x 25ml Glutamate Developer (Lyophilised) Red 1 vial Glutamate Enzyme Mix (Lyophilised) Green 1 vial Glutamate Standard (0.1M) Yellow 1 x 100µl
RelevanceGlutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Glutamate is the most abundant swift excitatory neurotransmitter in the mammalian nervous system. It is believed to be involved in learning and memory and has appeared to be involved in diseases like amyotrophic lateral sclerosis, lathyrism, autism, some forms of mental retardation and Alzheimer's disease. Glutamic acid is also present in a wide variety of foods, and has been used as a flavor enhancer in food industry.
ab83389 has been referenced in 29 publications.
- Wang K et al. Branched-chain amino acid aminotransferase 2 regulates ferroptotic cell death in cancer cells. Cell Death Differ 28:1222-1236 (2021). PubMed: 33097833
- Ito J et al. Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice. Elife 10:N/A (2021). PubMed: 33526170
- Abo El Gheit RE et al. Retinoprotective effect of agmatine in streptozotocin-induced diabetic rat model: avenues for vascular and neuronal protection : Agmatine in diabetic retinopathy. J Physiol Biochem 77:305-320 (2021). PubMed: 33635523
- Ohashi N et al. GLT1 gene delivery based on bone marrow-derived cells ameliorates motor function and survival in a mouse model of ALS. Sci Rep 11:12803 (2021). PubMed: 34140581
- Maier JP et al. Inhibition of metabotropic glutamate receptor III facilitates sensitization to alkylating chemotherapeutics in glioblastoma. Cell Death Dis 12:723 (2021). PubMed: 34290229