Glycerol-3-Phosphate Dehydrogenase (G3PDH) Assay Kit (Colorimetric) (ab174095)

Overview

  • Product name
    Glycerol-3-Phosphate Dehydrogenase (G3PDH) Assay Kit (Colorimetric)
  • Detection method
    Colorimetric
  • Sample type
    Cell Lysate, Tissue Lysate
  • Assay type
    Quantitative
  • Sensitivity
    < 1 mU/well
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammals
  • Product overview

    Glycerol-3-Phosphate Dehydrogenase (G3PDH) Assay Kit (Colorimetric) (ab174095) is a product where G3PDH reacts with the substrate to forms an intermediate, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and rapid and can detect Glycerol-3-Phosphate dehydrogenase activity less than 1 mU/well.

     

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glycerol-3-Phosphate Dehydrogenase (EC 1.1.1.8) is an important enzyme for lipid metabolism. It catalyzes the reversible conversion between dihydroxyacetone phosphate and glycerol-3-phosphate. GPDH plays multiple functions inside cells; it links carbohydrate and lipid metabolism, and provides electrons through the Glycerol-3-Phosphate Shuttle. When progenitor adipocytes differentiate into mature adipocytes, GPDH activity increases significantly. Analysis of glycerol-3-phosphate dehydrogenase activity is crucial for the study of fatty acid metabolic pathways.

  • Platform
    Microplate

Properties

Images

  • NADH measured in cell lysates showing quantity (nmol) per 1 mln cells after 30 min incubation time.

    Samples with the concentration of 5e6 cells/mL were used. Samples were diluted 2-18 fold.

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Measurement of Glycerol-3-Phosphate Dehydrogenase activity in 3T3-L1 pre- adipocyte (60 µg) and differentiated 3T3-L1 adipocytes (60 µg) using Glycerol-3-Phosphate Dehydrogenase activity (GPDH) Assay Kit (Colorimetric) (ab174095). This is example data only.

Protocols

References

ab174095 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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