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Glycogen Assay Kit II (Colorimetric) (ab169558) provides a simple, fast and robust way to measure Glycogen levels in various biological samples. This assay is suitable for measuring Glycogen levels in samples that contain reducing substances, which may interfere with the oxidase-based assays. In this assay, Glycogen is hydrolyzed into glucose, which is oxidized to form an intermediate that reduces a colorless probe to a colored product with strong absorbance at 450 nm. This high-throughput suitable assay kit can detect 4 - 40 µg/ml of Glycogen in samples.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
Glycogen serves as the main carbohydrate storage in animals and can be converted to glucose readily. It is primarily found in the liver and muscle tissues. Glycogen is a branched biopolymer comprising of α-1,4 linkage with α-1,6 linkages occurring every 8-10 glucose units along the backbone. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases.
|Development Enzyme Mix (lyophilized)||Green||1 vial|
|Glycogen Development Buffer||1 x 25ml|
|Glycogen Hydrolysis Buffer||1 x 25ml|
|Glycogen Standard (2.0 mg/ml)||Yellow||1 x 100µl|
|Hydrolysis Enzyme Mix (lyophilized)||Blue||1 vial|
Glycogen measured in cell lysates showing quantity (�g) per 1 mln cells.
Samples with the concentration of 1e7 cells/mL (HeLa) and 6.6e6 cells/mL (HCT116) were used. Samples were diluted 2-6 fold.
Glycogen measured in tissue lysates showing quantity (�g) per mg of extracted protein.
Protein concentration for samples varied from 25 mg/mL to 50 mg/mL. Samples were diluted 2-54 fold.
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