Anti-Glypican 4 antibody (ab118911)
Key features and details
- Rabbit polyclonal to Glypican 4
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glypican 4 antibody
See all Glypican 4 primary antibodies -
Description
Rabbit polyclonal to Glypican 4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide conjugated to KLH, corresponding to the C-terminal domain of Human Glypican 4.
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Positive control
- Human Liver and Human Pancreas tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (3) |
Use a concentration of 15 - 20 µg/ml.
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Notes |
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IHC-P
Use a concentration of 15 - 20 µg/ml. |
Target
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Function
Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system. -
Sequence similarities
Belongs to the glypican family. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 2239 Human
- Omim: 300168 Human
- SwissProt: O75487 Human
- Unigene: 58367 Human
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Alternative names
- dJ900E8.1 glypican 4 antibody
- Glypican proteoglycan 4 antibody
- Gpc4 antibody
see all
Images
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ab118911 staining Glypican in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Blue - nuclei (DAPI)
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ab118911, at 20 µg/ml, staining Glypican 4 in Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry.
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ab118911, at 20 µg/ml, staining Glypican 4 in Formalin-fixed, Paraffin-embedded Human Pancreas tissue by Immunohistochemistry.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab118911 has not yet been referenced specifically in any publications.