Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (Biotin), pre-adsorbed (ab6005)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (Biotin), pre-adsorbed
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy
Overview
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Product name
Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (Biotin), pre-adsorbed
See all IgG + IgM + IgA secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum. No reaction was observed against Human Serum proteins. This product is suitable for the detection of all Mouse immunoglobulin classes, isotypes and chain combinations. -
Tested applications
Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details -
Minimal
cross-reactivity
Human more details -
Immunogen
Mouse IgG, IgA and IgM whole molecules
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
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Purification notes
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/2000 - 1/20000.
|
|
Dot blot |
Use at an assay dependent dilution.
|
|
WB |
1/5000 - 1/20000.
|
|
ELISA |
1/1.2e+006.
|
|
IHC |
Use at an assay dependent dilution.
|
|
Immunomicroscopy |
Use at an assay dependent dilution.
|
Notes |
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ICC/IF
1/2000 - 1/20000. |
Dot blot
Use at an assay dependent dilution. |
WB
1/5000 - 1/20000. |
ELISA
1/1.2e+006. |
IHC
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab6005 has been referenced in 1 publication.
- Pahwa S et al. Characterization and regulation of MT1-MMP cell surface-associated activity. Chem Biol Drug Des N/A:N/A (2018). PubMed: 30480376