Goat F(ab')2 Anti-Rabbit IgG Fc (Biotin) (ab6021)
Key features and details
- Goat F(ab')2 Anti-Rabbit IgG Fc (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy, IP
Related conjugates and formulations
Overview
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Product name
Goat F(ab')2 Anti-Rabbit IgG Fc (Biotin)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Tested applications
Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy, IPmore details -
Immunogen
Rabbit IgG F(c) fragment
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
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Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab’)2 molecule -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000 - 1/5000.
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Dot blot |
Use at an assay dependent dilution.
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WB |
1/2000 - 1/10000.
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ELISA |
1/20000 - 1/100000.
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IHC |
Use at an assay dependent dilution.
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Immunomicroscopy |
Use at an assay dependent dilution.
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|
IP |
Use at an assay dependent dilution.
|
Notes |
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ICC/IF
1/1000 - 1/5000. |
Dot blot
Use at an assay dependent dilution. |
WB
1/2000 - 1/10000. |
ELISA
1/20000 - 1/100000. |
IHC
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent dilution. |
IP
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab6021 has been referenced in 1 publication.
- Lee JH et al. 53BP1 promotes ATM activity through direct interactions with the MRN complex. EMBO J : (2009). IP . PubMed: 20010693