Goat F(ab')2 Anti-Rabbit IgG Fc (FITC) (ab6018)
Key features and details
- Goat F(ab')2 Anti-Rabbit IgG Fc (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt
Related conjugates and formulations
Overview
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Product name
Goat F(ab')2 Anti-Rabbit IgG Fc (FITC)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(ab')2. -
Tested applications
Suitable for: ICC/IF, Immunomicroscopy, Flow Cytmore details -
Immunogen
Rabbit IgG F(c) fragment.
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
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Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 1.9 moles FITC per mole of Goat IgG F(ab')2 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/500 - 1/2500.
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Immunomicroscopy |
Use at an assay dependent dilution.
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Flow Cyt |
Use at an assay dependent dilution.
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Notes |
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ICC/IF
1/500 - 1/2500. |
Immunomicroscopy
Use at an assay dependent dilution. |
Flow Cyt
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab6018 has been referenced in 4 publications.
- Roy C et al. A role for ceruloplasmin in the control of human glioblastoma cell responses to radiation. BMC Cancer 22:843 (2022). PubMed: 35918659
- Antarianto RD et al. Hepatocyte Differentiation from iPSCs or MSCs in Decellularized Liver Scaffold: Cell-ECM Adhesion, Spatial Distribution, and Hepatocyte Maturation Profile. Organogenesis 18:2061263 (2022). PubMed: 35435152
- Roth H et al. Zika virus infection studies with CD34+ hematopoietic and megakaryocyte-erythroid progenitors, red blood cells and platelets. Transfusion 60:561-574 (2020). PubMed: 32086956
- Roussi S et al. Mitochondrial perturbation, oxidative stress and lysosomal destabilization are involved in 7beta-hydroxysitosterol and 7beta-hydroxycholesterol triggered apoptosis in human colon cancer cells. Apoptosis 12:87-96 (2007). PubMed: 17136497