Goat Anti-Human IgG Fc (HRP) (ab97225)

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Overview

  • Product name

    Goat Anti-Human IgG Fc (HRP)
    See all IgG secondary antibodies

  • Description

    Goat polyclonal Secondary Antibody to Human IgG - Fc (HRP)

  • Host species

    Goat

  • Target species

    Human

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.

  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details

  • Immunogen

    Other Immunogen Type corresponding to Human IgG.

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituents: 0.2% BSA, PBS, 0.00062% 5-chloro-2-methyl-4-isothiazolin-3-one, 0.00062% 2-methyl-4-isothiazolin-3-one
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

  • Conjugation notes

    Molar enzyme/ antibody protein ratio is 4:1

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary)
WB 1/2000 - 1/25000. Colormetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000

References

This product has been referenced in:

  • Stockdale L  et al. HIV, HCMV and mycobacterial antibody levels: a cross-sectional study in a rural Ugandan cohort. Trop Med Int Health 24:247-257 (2019). Read more (PubMed: 30506614) »
  • Avila S  et al. A differential sex-specific pattern of IgG2 and IgG4 subclasses of anti-drug antibodies (ADAs) induced by glatiramer acetate in relapsing-remitting multiple sclerosis patients. Mult Scler Relat Disord 34:92-99 (2019). Read more (PubMed: 31272071) »
See all 8 Publications for this product

Publishing research using ab97225? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Thank you for your prompt response.

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Answer

I'm happy to help! Please contact me again if you need anything further.

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Question

I would like a Certificate of Compliance for ab97225, lot GR68321.

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Answer

Thanks for your enquiry. Attached please find the CoC.

I hope this is helpful. Please contact me again if you have any further questions.

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Question

We have bought the following conjugates from your company. We asked the agancy that we bought about the temperature to keep them to increase the time of keeping. They told us to keep them in -20 C0 and we did for two weeks up to now, but now when we checked your website information it is recommended to be kept in 4 degree castigates.

Please let me know if keeping them in -20 C0 has made any problem for them and can we continue to keep them in this temperature or not?

Goat-polyclonal-Secondary-Antibody-to-Human-IgG-Fc-HRP(ab97225)

Goat-polyclonal-Secondary-Antibody-to-Bird-IgG-H-L-HRP(ab112773)

Goat-polyclonal-Secondary-Antibody-to-Horse-IgG-H-L-HRP(ab102396)

Goat-polyclonal-Secondary-Antibody-to-Syrian-Hamster-IgG-H-L(HRP-ab6892)

Goat-polyclonal-Secondary-Antibody-to-Human-IgM-mu-chain-HRP(ab97205)

Goat-polyclonal-Secondary-Antibody-to-Cow-IgG-H-L-HRP(ab6934)

Rabbit-polyclonal-Secondary-Antibody-to-Sheep-IgG-Fc-HRP (ab102299)

Rabbit-polyclonal-Secondary-Antibody-to-Goat-IgG-Fc-HRP-pre-adsorbed (ab102345)

Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fc-HRP (ab97265)

Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-Fc-HRP-pre-adsorbed (ab97090)


Best,

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Answer

Thank you for contacting us.

The datasheet of these antibodies say "Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles"

This mean after receiving the antibody you can keep antibody at 4C for 1-2 weeks when using in your experiments. After you finish your experiment we recomendaliquoting the antibody 5-10 ul in vialsand storing at -20C. We suggest this to avoidthe extra freeze thawing steps, every time you use the antibody.

If you have already stored it at -20C, this is absolutely fine!

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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