Goat Anti-Human IgG Fc (HRP) (ab97225)

Submit a review Q&A (3)References (5)

Overview

  • Product name
    Goat Anti-Human IgG Fc (HRP)
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Human IgG - Fc (HRP)
  • Host species
    Goat
  • Target species
    Human
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Immunogen

    Other Immunogen Type corresponding to Human IgG.

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes
    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary)
WB 1/2000 - 1/25000. Colormetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000

References

This product has been referenced in:
  • Liu Y  et al. Binding of EphrinA5 to RET receptor tyrosine kinase: An in vitro study. PLoS One 13:e0198291 (2018). Read more (PubMed: 29889908) »
  • Marino N  et al. Development of a versatile oncolytic virus platform for local intra-tumoural expression of therapeutic transgenes. PLoS One 12:e0177810 (2017). Read more (PubMed: 28542292) »
See all 5 Publications for this product

Publishing research using ab97225? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Thank you for your prompt response.

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Answer

I'm happy to help! Please contact me again if you need anything further.

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Question

I would like a Certificate of Compliance for ab97225, lot GR68321.

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Answer

Thanks for your enquiry. Attached please find the CoC.

I hope this is helpful. Please contact me again if you have any further questions.

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Question

We have bought the following conjugates from your company. We asked the agancy that we bought about the temperature to keep them to increase the time of keeping. They told us to keep them in -20 C0 and we did for two weeks up to now, but now when we checked your website information it is recommended to be kept in 4 degree castigates.

Please let me know if keeping them in -20 C0 has made any problem for them and can we continue to keep them in this temperature or not?

Goat-polyclonal-Secondary-Antibody-to-Human-IgG-Fc-HRP(ab97225)

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Goat-polyclonal-Secondary-Antibody-to-Horse-IgG-H-L-HRP(ab102396)

Goat-polyclonal-Secondary-Antibody-to-Syrian-Hamster-IgG-H-L(HRP-ab6892)

Goat-polyclonal-Secondary-Antibody-to-Human-IgM-mu-chain-HRP(ab97205)

Goat-polyclonal-Secondary-Antibody-to-Cow-IgG-H-L-HRP(ab6934)

Rabbit-polyclonal-Secondary-Antibody-to-Sheep-IgG-Fc-HRP (ab102299)

Rabbit-polyclonal-Secondary-Antibody-to-Goat-IgG-Fc-HRP-pre-adsorbed (ab102345)

Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fc-HRP (ab97265)

Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-Fc-HRP-pre-adsorbed (ab97090)


Best,

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Answer

Thank you for contacting us.

The datasheet of these antibodies say "Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles"

This mean after receiving the antibody you can keep antibody at 4C for 1-2 weeks when using in your experiments. After you finish your experiment we recomendaliquoting the antibody 5-10 ul in vialsand storing at -20C. We suggest this to avoidthe extra freeze thawing steps, every time you use the antibody.

If you have already stored it at -20C, this is absolutely fine!

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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