Goat Anti-Human IgG Fc (HRP) (ab97225)
Key features and details
- Goat Anti-Human IgG Fc (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Related conjugates and formulations
Overview
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Product name
Goat Anti-Human IgG Fc (HRP)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other IgM, IgA, and light chains is less than 1%. This antibody may cross react with IgG from other species.
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Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Immunogen
Other Immunogen Type corresponding to Human IgG.
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.80
Constituents: 0.2% BSA, 99% PBS, 0.05% CMIT/MIT based preservative -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
Conjugation notes
Molar enzyme/ antibody protein ratio is 4:1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent dilution.
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| IHC-P |
1/200 - 1/2000.
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| ELISA |
1/10000 - 1/100000. (Primary)
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| WB |
1/2000 - 1/25000. Colormetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000
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| Notes |
|---|
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ICC
Use at an assay dependent dilution. |
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IHC-P
1/200 - 1/2000. |
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ELISA
1/10000 - 1/100000. (Primary) |
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WB
1/2000 - 1/25000. Colormetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000 |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab97225 has been referenced in 21 publications.
- Demers-Mathieu V et al. Previous viral symptoms and individual mothers influenced the leveled duration of human milk antibodies cross-reactive to S1 and S2 subunits from SARS-CoV-2, HCoV-229E, and HCoV-OC43. J Perinatol 41:952-960 (2021). PubMed: 33649442
- Kim YJ et al. Neutralizing Human Antibodies against Severe Acute Respiratory Syndrome Coronavirus 2 Isolated from a Human Synthetic Fab Phage Display Library. Int J Mol Sci 22:N/A (2021). PubMed: 33671877
- Claro F et al. Immunoglobulin G antibody response to the Sputnik V vaccine: previous SARS-CoV-2 seropositive individuals may need just one vaccine dose. Int J Infect Dis 111:261-266 (2021). PubMed: 34343704
- Stockdale L et al. Cytomegalovirus Antibody Responses Associated With Increased Risk of Tuberculosis Disease in Ugandan Adults. J Infect Dis 221:1127-1134 (2020). PubMed: 31689350
- Thakkar D et al. 10D1F, an Anti-HER3 Antibody that Uniquely Blocks the Receptor Heterodimerization Interface, Potently Inhibits Tumor Growth Across a Broad Panel of Tumor Models. Mol Cancer Ther 19:490-501 (2020). PubMed: 31911530