Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed (ab47830)
Key features and details
- Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF
Overview
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Product name
Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed
See all IgG + IgM secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse IgM. -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Minimal
cross-reactivity
Cow, Horse, Human more details -
Immunogen
Full length native protein (purified) (Mouse)
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
IgG and protease free -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
This antibody was labelled with fluorescein isothiocyanate (FITC, MW 390 da) Fluorochrome/Protein Ratio: 3.8 moles FITC per mole of Goat IgGAbsorption Wavelength: 495 nm Emission Wavelength: 528 nm -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt | ||
ICC/IF |
Notes |
---|
IF (microscopy): 1/500 - 1/2500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (7)
ab47830 has been referenced in 7 publications.
- Zhou F et al. Suppression of estrogen receptor-beta promotes gastric cancer cell apoptosis with induction of autophagy. Am J Transl Res 12:4397-4409 (2020). PubMed: 32913514
- Koiwai K et al. Improvement of Production and Isolation of Human Neuraminidase-1 in Cellulo Crystals. ACS Appl Bio Mater 2:4941-4952 (2019). PubMed: 35021494
- Kitakaze K et al. Combined replacement effects of human modified ß-hexosaminidase B and GM2 activator protein on GM2 gangliosidoses fibroblasts. Biochem Biophys Rep 7:157-163 (2016). PubMed: 28955902
- Jiang LY et al. Cystic fibrosis transmembrane conductance regulator is correlated closely with sperm progressive motility and normal morphology in healthy and fertile men with normal sperm parameters. Andrologia 46:824-30 (2014). PubMed: 23998339
- Li CY et al. CFTR is essential for sperm fertilizing capacity and is correlated with sperm quality in humans. Hum Reprod 25:317-27 (2010). ICC/IF . PubMed: 19923167
- Murphy MJ et al. Essential role for Pbx1 in corneal morphogenesis. Invest Ophthalmol Vis Sci 51:795-803 (2010). PubMed: 19797217
- Chen WY et al. Cl- Is Required for HCO3- Entry Necessary for Sperm Capacitation in Guinea Pig: Involvement of a Cl-/HCO3- Exchanger (SLC26A3) and CFTR. Biol Reprod : (2008). ICC/IF . PubMed: 18784352